The CCDC97 Knockout HeLa Polyclonal Cells are a CRISPR/Cas9-mediated gene-disrupted polyclonal population in the HeLa human cervical adenocarcinoma cell line, targeting the CCDC97 locus. This polyclonal knockout model offers a heterogeneous cell population for loss-of-function studies without the biases of clonal isolation, ensuring that the functional consequences of CCDC97 disruption are assessed across a diverse genetic background. The product is designed for researchers investigating centrosome biology, ciliogenesis, and related signaling pathways.
HeLa cells are an immortalized epithelial cell line originally derived from a patient with cervical adenocarcinoma. These cells are a cornerstone of biomedical research, particularly in cancer biology, owing to their robust growth, extensive characterization, and aberrant centrosome amplification, which makes them an ideal host for studying centrosome-associated proteins like CCDC97.
CCDC97 encodes a coiled-coil domain-containing protein that localizes to centriolar satellites and is essential for primary cilium formation and ciliary trafficking. It interacts with components of the centriolar satellite machinery, including CEP290, PCM1, and OFD1, and associates with the BBSome complex. Upstream regulators include FOXJ1, RFX2, and RFX3 transcription factors, while downstream targets encompass GLI1, GLI2, and Cyclin D1. CCDC97 disruption impairs Hedgehog and Wnt signal transduction, leading to altered expression of IFT88 and ARL13B, and compromised ciliary function.
In the HeLa cell context, loss of CCDC97 disrupts primary cilium assembly and perturbs Hedgehog and Wnt signaling cascades that regulate proliferation, migration, and cell polarity. This model allows investigation of how ciliary defects influence cervical cancer cell behavior, including cell cycle progression, invasiveness, and drug sensitivity. The polyclonal nature captures population-level phenotypic heterogeneity, enhancing the translational relevance of the findings.
Key applications include immunofluorescence microscopy for acetylated ??-tubulin to assess ciliogenesis, Western blotting for Hedgehog pathway components such as GLI1 and SMO, cell proliferation assays, Transwell migration and invasion assays, and flow cytometric analysis of cell cycle distribution. The cells are also valuable for drug screening targeting centrosome or cilium pathways. For additional information or customized inquiries, please contact Ascent Research.
