The CCDC97 Knockout SK-HEP-1 Polyclonal Cells product consists of a CRISPR/Cas9-edited polyclonal knockout cell population in which the human CCDC97 gene has been disrupted in the SK-HEP-1 cell line. This heterogeneous pool of gene-edited cells circumvents clonal selection biases and provides a robust loss-of-function model for investigating CCDC97 biological roles in a liver adenocarcinoma background.
SK-HEP-1 is a human liver adenocarcinoma cell line originally established from the ascitic fluid of a 56-year-old male patient. It displays epithelial characteristics and is widely employed as a model for hepatocellular carcinoma (HCC), enabling research into tumorigenic mechanisms, metastatic behavior, and pharmacological responses.
CCDC97 (coiled-coil domain-containing 97) encodes a protein that directly binds the dynein light chain subunit DYNLT1 and is proposed to act as a dynein-interacting factor. This association integrates CCDC97 into the cytoplasmic dynein motor complex, where it facilitates retrograde transport and contributes to ciliary assembly and maintenance. CCDC97 functions within a network that includes dynein intermediate chains, ciliary transition zone proteins, and axonemal components, and its expression may be regulated by RFX transcription factors. Downstream, loss of CCDC97 is predicted to impair DYNLT1-mediated processes, disrupting ciliogenesis and intracellular trafficking, with consequent effects on cell cycle progression and signal transduction.
In SK-HEP-1 cells, which originate from hepatic adenocarcinoma, CCDC97 disruption offers a unique tool to examine the intersection of dynein transport, cilia biology, and hepatocellular carcinoma. Although hepatocytes and many liver cancer cell lines are not typically ciliated, ciliary proteins can influence cancer cell migration, invasion, and proliferation through non-canonical mechanisms. This polyclonal knockout model permits interrogation of how dynein-related functions impinge on HCC phenotypes, including potential modulation of sorafenib sensitivity and gene expression changes associated with epithelial-mesenchymal transition.
The CCDC97 knockout polyclonal cells are suited for diverse experimental applications, including immunofluorescence staining for ciliary markers (acetylated ??-tubulin, Arl13b), co-immunoprecipitation of dynein complex components, and RT-qPCR profiling of ciliogenesis-associated genes. Functional assays such as flow-cytometric cell cycle analysis, transwell migration and invasion studies, and drug sensitivity testing with sorafenib can further define the phenotypic consequences of CCDC97 loss. The heterogeneous population is also amenable to high-throughput screening for ciliopathy modifiers and transcriptome-wide RNA-seq analysis. For additional product details or technical inquiries, please contact Ascent Research.
