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Home / Products / Genome-edited Cells / Knockout Cell Lines / CCL7 Knockout UMUC-3 Polyclonal Cells

Cat. No. ARG43203

CCL7 Knockout UMUC-3 Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Urinary bladder
Disease:
Carcinoma

Datasheet

CRISPR/Cas9-edited polyclonal knockout cell population with CCL7 gene disruption, generated in the Ca Ski human cervical carcinoma cell line (HPV-16 positive, metastatic small intestine origin). This model eliminates MCP-3/CCL7 chemokine secretion, disrupting downstream signaling through receptors CCR1, CCR2, CCR3, and CCR5, as well as associated JAK/STAT, MAPK, and PI3K/AKT pathways. Ideal for investigating tumor-immune cell interactions, chemokine-mediated monocyte/macrophage recruitment, and inflammatory paracrine signaling in cervical cancer. Enables functional studies via Transwell migration assays, ELISA, phospho-protein profiling, and co-culture immune chemotaxis experiments, supporting drug target validation and microenvironment research.

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Shipping Info:

Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

UM-UC-3

Age

Unknown

Derived From Site

In situ; Urinary bladder

Gene Name

CCL7

Gene Identifier

NCBI Gene ID 6354

Morphology

Epithelial-like

Growth Mode

Adherent

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

RPMI 1640

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

The CCL7 Knockout Ca Ski Polyclonal Cells represent a CRISPR/Cas9-edited, polyclonal knockout cell population in which the CCL7 gene has been disrupted via targeted genome editing.

This product is supplied as a heterogeneous pool of cells harboring diverse loss-of-function mutations in the CCL7 locus, providing a robust model for studying CCL7-dependent processes without the clonal selection biases inherent in monoclonal knockout lines.

The polyclonal format ensures representation of multiple editing events, minimizing the impact of off-target effects and better reflecting the complexity of gene loss in a mixed population.

Cells are derived from the Ca Ski parent line and maintain key characteristics of the host cell type, including human papillomavirus (HPV-16) positivity and epithelial morphology, allowing direct phenotypic comparisons between wild-type and CCL7-deficient states.

The product is suitable for a broad range of functional, biochemical, and immuno-oncological assays requiring ablation of MCP-3/CCL7 chemokine activity.