The CCNYL1 Knockout AGS Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population of the AGS human gastric adenocarcinoma cell line. This product provides a loss-of-function model for the putative cyclin CCNYL1, enabling investigation of its roles in cell cycle progression and Wnt/??-catenin signaling. The polyclonal knockout pool maintains the heterogeneous background of the parental line while disrupting CCNYL1, offering a robust system for studying gene function in a gastric cancer context.
Derived from a poorly differentiated gastric adenocarcinoma, the AGS cell line is an epithelial model widely used in gastric cancer research. AGS cells retain key features of advanced adenocarcinomas, including deregulated proliferation and survival signaling. In the context of CCNYL1 knockout, this background provides a physiologically relevant platform to dissect cell cycle control and Wnt pathway contributions to gastric tumorigenesis.
CCNYL1 is a putative cyclin predicted to interact with CDK kinases, particularly CDK14, CDK2, and CDK5. These complexes may phosphorylate downstream targets such as RB1 and the Wnt co-receptor LRP6. RB1 phosphorylation releases E2F transcription factors to promote expression of cyclins E1 and D1, facilitating G1/S transition. LRP6 phosphorylation, in turn, stabilizes ??-catenin and activates Wnt target genes. CCNYL1 expression is regulated by cell cycle-dependent transcription factors and mitogenic signals, positioning it at the interface of proliferation and Wnt/??-catenin signaling.
Disruption of CCNYL1 in AGS cells is expected to impair G1/S progression and alter cell cycle distribution. Given the frequent hyperactivation of cell cycle machinery and Wnt signaling in gastric adenocarcinoma, this knockout model enables dissection of pathway crosstalk without clonal selection artifacts. The polyclonal population offers a representative view of tumor cell responses and can be used to investigate dependency on CCNYL1-mediated signaling and potential synthetic lethal interactions.
The product supports assays such as flow cytometry for cell cycle profiling, BrdU or MTT proliferation assays, and western blotting for phosphorylated RB1 and Cyclin D1. Wnt pathway activity can be measured using TOP/FOP Flash reporter assays, and colony formation assays assess tumorigenic potential. The knockout cells are also suitable for functional genomics screens and evaluating CDK inhibitor sensitivity. For technical inquiries, contact Ascent Research.
