The CCNYL1 Knockout HEK293T Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout population of HEK293T cells with targeted disruption of the CCNYL1 gene. This polyclonal pool provides a loss-of-function model for investigating Cyclin-Y-like 1 protein function in a human cellular background. By preserving genetic heterogeneity, the mixed population helps avoid clonal artifacts and may reveal a spectrum of functional consequences. The cells are suitable for direct use in downstream molecular and cell-based assays.
HEK293T is a widely used human embryonic kidney cell line that stably expresses the SV40 large T antigen, enabling high-level transient expression and episomal replication of plasmids bearing the SV40 origin. This feature makes the line a preferred host for recombinant protein production, viral packaging, and signal transduction studies. Its ease of transfection and robust growth provide a consistent and well-characterized platform for functional genomics and drug discovery applications.
CCNYL1 encodes Cyclin-Y-like 1, a predicted cyclin that interacts with cyclin-dependent kinases including CDK2, CDK14, and CDK16 to modulate cell cycle progression. It has also been implicated in Wnt/??-catenin signaling through association with the co-receptor LRP6, promoting ??-catenin stabilization and TCF/LEF-mediated transcription. Downstream, CCNYL1 may influence the activity of cell cycle effectors such as RB1 and E2F. Knockout of CCNYL1 likely disrupts the coordination between cyclin-CDK complexes and the Wnt pathway, leading to impaired cell cycle control and altered gene expression profiles.
In the HEK293T background, loss of CCNYL1 is expected to perturb both cell proliferation and Wnt/??-catenin transcriptional output. This model is particularly valuable for studying the intersection of cyclin function and Wnt signaling in an epithelial context, with relevance to cancer biology and neurodevelopmental disorders. Although the presence of SV40 large T antigen may alter cell cycle regulation, the system remains an accessible tool for mechanistic dissection and initial functional screening of the CCNYL1 pathway.
Researchers can apply this knockout model in diverse assays including Western blotting and RT-qPCR for protein and mRNA analysis, flow cytometry and BrdU incorporation for cell cycle profiling, and TOPFlash luciferase reporters for Wnt pathway activity. Co-immunoprecipitation studies can probe CCNYL1 interactions with CDK2, CDK14, LRP6, or ??-catenin. The polyclonal format is also suitable for high-throughput screening of modulators targeting cyclin-CDK or Wnt signaling. For further information, please contact Ascent Research.
