CCPG1 Knockout Huh-7 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal cell population derived from the Huh-7 hepatocellular carcinoma line, featuring targeted disruption of the CCPG1 gene. This loss-of-function model avoids clonal selection, providing a heterogeneous knockout pool that maintains parental growth properties. Supplied as early-passage frozen vials, the cells are ready for expansion and retain epithelial morphology suitable for diverse in vitro applications.
The Huh-7 cell line, established in 1982 from a 57-year-old Japanese male liver tumor, is a well-differentiated hepatocellular carcinoma model widely used for hepatic metabolism, HCV replication, and liver cancer research. Exhibiting hepatocyte features including liver-specific enzyme expression, Huh-7 supports robust HCV replication and harbors wild-type TP53, enabling investigation of p53-dependent pathways. In this context, CCPG1 knockout facilitates dissection of p53-mediated tumor suppressor networks.
CCPG1 is a p53/p73 target gene induced by genotoxic stress that enforces G2/M cell cycle arrest by inhibiting cyclin B1 (CCNB1)?CCDK1 activity. Its protein product interacts with p53, potentially stabilizing the tumor suppressor and enhancing growth inhibition. Upstream, ATM/ATR kinases sense DNA damage and activate p53, while downstream, CCPG1 cooperates with CDKN1A to halt cell cycle progression. Additionally, CCPG1 binds hepatitis delta antigen (HDAg), linking it to antiviral defense.
In hepatocellular carcinoma, CCPG1 loss may disrupt p53-dependent growth control, promoting hepatocarcinogenesis. The Huh-7 background, with intact p53 and liver-specific signaling, provides a physiologically relevant system to assess how CCPG1 deletion alters cell cycle, DNA damage responses, and tumorigenic behavior. This model is particularly suited for exploring the interplay between viral factors like HDAg and host cell cycle checkpoints in liver cancer.
This knockout model supports multiple research applications, including tumor suppressor studies, DNA damage response analysis, and antiviral research. Typical assays include Western blotting and RT-qPCR for gene expression profiling, flow cytometry for cell cycle analysis, and colony formation assays to assess proliferative changes. Furthermore, the cells are suitable for DNA damage induction experiments using etoposide and for co-immunoprecipitation and ChIP-qPCR to examine CCPG1?Cp53 interactions and chromatin binding. For detailed technical information or to inquire about customized applications, please contact Ascent Research.
