The CD164 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population engineered for loss-of-function studies of the CD164 gene in a human embryonic kidney epithelial background. This product offers a heterogeneous knockout cell pool, enabling robust functional interrogation of CD164 without the limitations of clonal selection. The targeted disruption of CD164 abolishes expression of the encoded sialomucin adhesion receptor, providing a versatile model for investigating its roles in cell adhesion, migration, and signal transduction.
HEK293T cells are a widely characterized host line derived from human embryonic kidney epithelial cells and stably express the SV40 large T antigen. This feature facilitates high-efficiency transfection and lentiviral packaging, rendering the line a workhorse for recombinant protein expression and gene-editing applications. The cells exhibit adherent epithelial morphology and retain key signaling networks, making them a suitable platform for studying the molecular mechanisms of CD164 in a controlled in vitro setting.
CD164 is a cell adhesion sialomucin and co-receptor that modulates migration, proliferation, and stem cell homing. It interacts with CXCR4 and ??1 integrin and is activated by CXCL12/SDF-1, triggering MAPK/ERK signaling and integrin activation. Transcriptional regulation involves GATA factors and TGF-??, while cross-talk with Wnt/??-catenin influences ??-catenin/TCF/LEF activity. In myogenic programs, CD164 promotes MyoD and myogenin expression, placing it at the intersection of hematopoietic homing, cancer metastasis, and development.
Disruption of CD164 in HEK293T cells impairs adhesion and migration, as the polyclonal knockout lacks the wild-type receptor. The epithelial origin and partial mesenchymal traits of these cells allow dissection of CD164-dependent adhesion mechanisms and CXCR4/CXCL12 signaling dynamics. This model enables examination of compensatory changes in Wnt/??-catenin pathway activity, providing a practical tool for studying CD164 in epithelial?Cmesenchymal transition and hematopoietic niche interactions.
Research applications include functional analyses of cell adhesion and transwell migration, investigation of CD164 in cancer metastasis, hematopoietic stem cell homing assays, drug target validation, and protein?Cprotein interaction studies via co-immunoprecipitation. Representative methodologies encompass Western blotting, RT-qPCR, flow cytometry, and Wnt/??-catenin reporter assays to assess pathway activity. For technical specifications, protocols, or ordering information, contact Ascent Research.
