The CD164L2 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population targeting the CD164L2 gene in the human near-haploid HAP1 cell line. This heterogeneous pool of indel-bearing cells provides a loss-of-function model for studying CD164L2, a sialomucin involved in cell adhesion, proliferation, and endolysosomal sorting. The polyclonal format avoids clonal bias, enabling robust screening and initial functional characterization. CRISPR/Cas9-mediated gene disruption ensures stable ablation of CD164L2 expression, creating a reliable platform for downstream assays.
HAP1 cells originate from the KBM-7 chronic myeloid leukemia line and possess a near-haploid karyotype, which simplifies knockout generation and genotype-phenotype correlations. These adherent cells retain leukocyte-related signaling pathways and are widely used for CRISPR-based functional genomics. The CD164L2 knockout in HAP1 thus leverages a genetically tractable background with hematopoietic relevance, facilitating studies of adhesion and migration in a disease-relevant context.
CD164L2, also termed endolyn, operates at the interface of the plasma membrane and endolysosomal system. It interacts with integrin beta1 to activate FAK/Src signaling, promoting actin remodeling through paxillin and modulating cell adhesion and migration. Intracellularly, CD164L2 engages Rab7 and ESCRT machinery, mediating endosomal sorting and lysosomal trafficking as indicated by LAMP1 co-localization. These dual functions position CD164L2 as a coordinator of extracellular matrix sensing and intracellular trafficking.
In HAP1 cells, the CD164L2 knockout permits unambiguous dissection of its roles in leukocyte-associated adhesive and migratory processes, free from second-allele interference. The polyclonal knockout population mirrors the genetic heterogeneity of gene disruption, minimizing clone-specific artifacts. This model is particularly suited to exploring CD164L2 function in hematopoietic cell biology, where sialomucins influence immune cell homing and potential malignant dissemination.
Standard applications include Western blotting and immunofluorescence to verify loss of expression, cell adhesion and transwell migration assays to quantify functional changes, and flow cytometry for surface receptor profiling. Co-immunoprecipitation can assess interactions with integrins or Rab proteins, while endocytosis assays monitor trafficking alterations. This knockout product supports cancer metastasis research and broader investigations of CD164L2-dependent mechanisms. For further information, contact Ascent Research.
