The CD180 Knockout HAP1 Polyclonal Cells product comprises a CRISPR/Cas9-edited polyclonal knockout cell population derived from HAP1 cells, in which the CD180 gene has been disrupted to generate a loss-of-function model. This polyclonal population provides a genetically heterogeneous pool of cells carrying diverse edits at the target locus, enabling robust functional genomics studies without the need for single-cell cloning. The knockout model is designed for investigations into CD180-dependent signaling mechanisms and their roles in immune regulation, particularly within the context of TLR4-mediated pathways.
The HAP1 host cell line is a near-haploid human cell line originally derived from the KBM-7 chronic myeloid leukemia background. As a haploid model, HAP1 cells facilitate straightforward gene perturbation and are widely employed in genetic screens and pathway dissection. Their human origin and stable growth characteristics make them a versatile platform for studying signal transduction, protein interactions, and drug responses in a simplified genomic setting. This cell line retains expression of key components of innate immune signaling, allowing functional interrogation of immune receptors such as CD180.
CD180 (also known as RP105) is a toll-like receptor family member that lacks an intracellular TIR domain and functions as an accessory modulator of TLR4 signaling. It forms a cell surface complex with MD-1, a secreted cofactor, and this heterodimer interacts with TLR4 to regulate ligand responsiveness. Upon stimulation by microbial ligands such as LPS, CD180/MD-1 influences downstream activation of MyD88-dependent and -independent cascades, ultimately modulating NF-??B and MAPK pathways. These signaling events control B cell proliferation and antibody secretion, positioning CD180 as a critical checkpoint in innate and adaptive immune responses.
In the HAP1 cellular context, disruption of CD180 allows dissection of its specific contributions to TLR4 signal transduction without interference from intact B cell-specific processes. Because HAP1 cells express core TLR4 pathway components including TLR4, MD-1, MyD88, and NF-??B, the knockout model provides a genetically tractable system to analyze CD180??s regulatory role. This model is especially valuable for mapping protein?Cprotein interactions between CD180/MD-1 and TLR4, and for examining how loss of CD180 alters downstream signaling kinetics and gene expression programs normally associated with immune activation.
Typical research applications of the CD180 Knockout HAP1 Polyclonal Cells include mechanistic studies of TLR4 signaling, functional screening in autoimmune disease models, and investigation of B cell-related malignancies such as lymphomas and chronic lymphocytic leukemia. The polyclonal population is well-suited for lentiviral CRISPR screens, co-immunoprecipitation assays to probe MD-1 or TLR4 interactions, luciferase-based reporter assays for NF-??B activity, and flow cytometric analyses of surface receptor expression. Researchers in immunology, signal transduction, and drug discovery will find this model a valuable tool for exploring CD180-dependent regulatory networks and for identifying therapeutic targets. For further details, please contact Ascent Research.
