CD2AP Knockout 786-O Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population designed to disrupt the CD2AP gene in Homo sapiens. This population model offers a heterogeneous loss-of-function system, eliminating clonal bias while enabling robust investigation of CD2AP-dependent processes. The polyclonal pool is generated by CRISPR/Cas9-mediated gene disruption, providing a versatile tool for studying gene function at the population level.
The host 786-O cell line originates from a primary clear cell renal cell adenocarcinoma and is a well-characterized model for clear cell renal cell carcinoma (ccRCC). These adherent epithelial cells are widely employed to study renal cancer biology, providing a relevant platform for dissecting the molecular mechanisms underlying tumor progression and cytoskeletal dynamics.
CD2AP functions as an adaptor protein linking membrane receptors to the actin cytoskeleton, essential for podocyte slit diaphragm integrity and T-cell receptor (TCR) signaling. Its activity is regulated by TCR activation and nephrin clustering, followed by phosphorylation by Src family kinases. CD2AP interacts with nephrin, podocin, cortactin, c-Cbl, and the PI3K p85 subunit, coordinating downstream events such as actin polymerization, stabilization of cell adhesion complexes, and endocytic vesicle formation. Through these interactions, CD2AP integrates signals from the nephrin?CNEPH1?Cpodocin complex to regulate actin remodeling and endocytosis.
In 786-O ccRCC cells, CD2AP knockout disrupts the receptor?Ccytoskeleton linkage, potentially altering cell adhesion, migration, and invasion. This polyclonal knockout model mitigates single-clone artifacts, offering a physiologically relevant average response. Given CD2AP??s association with mTOR signaling and endocytic trafficking, its loss may impact growth factor signaling and nutrient sensing, pathways frequently dysregulated in renal carcinoma.
Applications include western blotting and RT-qPCR for knockout validation, immunofluorescence for actin cytoskeleton analysis, and functional assays such as migration and adhesion tests. Co-immunoprecipitation can be used to assess interactions among CD2AP, nephrin, podocin, cortactin, and c-Cbl. The polyclonal nature supports population-level studies of CD2AP??s role in actin dynamics, cell adhesion, and endocytosis in ccRCC. For further details, please contact Ascent Research.
