The CD300LB Knockout HAP1 Polyclonal Cells product is a CRISPR/Cas9-edited polyclonal knockout cell population in which the CD300LB gene has been disrupted to generate a loss-of-function model for this activating immune receptor. This heterogeneous pool, derived from the near-haploid HAP1 cell line, carries diverse edits at the CD300LB locus, enabling robust functional analyses of CD300LB-dependent signaling pathways. The polyclonal format reduces clonal artifacts and provides a more representative gene disruption model.
The HAP1 host cell line is an adherent, fibroblast-like cell line with a predominantly haploid karyotype, derived from KBM-7 chronic myeloid leukemia cells. Its near-haploid genome simplifies gene editing and genotype-phenotype correlations, making it ideal for functional genomics and genetic screening. Retaining myeloid lineage features, HAP1 cells offer a relevant background for studying immune receptors like the CD300 family without diploid complexity.
CD300LB is an activating immunoreceptor on myeloid cells that binds phosphatidylserine and other aminophospholipids on apoptotic cells, debris, and activated platelets. Ligand binding phosphorylates DAP12, which recruits and activates Syk kinase. Syk activates PI3K, PLC??, and PKC, leading to calcium flux, MAP kinase and NF-??B activation, and phagocytosis and release of cytokines like TNF-??, IL-6, IL-10. Co-engagement with Fc?? receptors and IgG immune complexes modulates signaling, making CD300LB a key regulator of efferocytosis and innate immunity.
In HAP1 cells, CD300LB disruption abolishes aminophospholipid-driven signaling, providing a clean loss-of-function system to dissect phagocytosis and cytokine release. The haploid genome ensures phenotypes are directly tied to CD300LB deficiency. This model is well-suited for examining the CD300LB?CDAP12?CSyk axis, comparing lipid-induced and Fc receptor pathways, and studying receptor cross-talk.
This knockout population supports quantitative phagocytosis and efferocytosis assays by flow cytometry or confocal microscopy, cytokine profiling (e.g., TNF-??, IL-6, IL-10) by ELISA, and analysis of Syk phosphorylation and calcium mobilization. It is also valuable for genetic interaction screens, target validation in autoimmune diseases like lupus and allergic inflammation, and transcriptomic studies (RNA-seq). For technical support, please contact Ascent Research.
