The CD300LD Knockout TE1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population derived from the TE1 human esophageal squamous cell carcinoma line, targeting the CD300LD gene. This model eliminates CD300LD protein expression, providing a clean genetic system to study receptor function without compensatory antibody interference. The polyclonal pool ensures representation of diverse editing events, avoiding clonal artifacts and maintaining robustness in population-based assays.
The TE1 cell line originates from a well-differentiated squamous cell carcinoma of the esophagus and is a standard model in cancer research. Its epithelial origin and stable growth characteristics make it ideal for investigating tumor biology, drug responses, and signaling pathways. This background allows integration of CD300LD knockout studies into established esophageal cancer experimental frameworks, linking immune receptor function to epithelial malignancies.
CD300LD is an activating immune receptor that associates with the ITAM-containing adaptor DAP12. Ligand engagement, such as by phosphatidylserine, induces DAP12 phosphorylation and subsequent activation of Syk kinase. This triggers the PI3K-AKT and NF-??B pathways, leading to phagocytosis and secretion of pro-inflammatory cytokines including TNF-?? and IL-1??. The pathway is modulated by Lyn kinase and other interacting adaptors, which collectively regulate myeloid cell activation and inflammatory responses.
Disruption of CD300LD in TE1 cells facilitates the study of this receptor??s potential roles in cancer, where its expression may influence tumor cell signaling and immune evasion. CD300LD knockout can reveal contributions to cytokine production, phagocytic capacity, and downstream activation of NF-??B or PI3K-AKT, all relevant to the tumor microenvironment. This model is thus valuable for exploring whether CD300LD serves as a tumor-intrinsic immune checkpoint or modulator of cancer-related inflammation.
These cells are applicable in immune checkpoint research, phagocytosis studies, and inflammatory disease modeling. Functional readouts include phagocytosis assays, cytokine secretion measurements, and phospho-Syk analysis via western blotting or flow cytometry. Gene expression analysis by RT-qPCR and protein detection by flow cytometry further validate knockout efficiency. For inquiries, please contact Ascent Research.
