The CD3E Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited HAP1 cell population with targeted CD3E gene disruption. Supplied as a polyclonal pool, this heterogeneous loss-of-function model avoids clonal biases and provides a versatile tool for studying CD3E in a human myeloid leukemia background.
HAP1 is a near-haploid human cell line derived from KBM-7 chronic myeloid leukemia cells. Originating from a male donor, these adherent cells have a predominantly haploid karyotype, enabling clear genotype-phenotype relationships. Widely used in haploid genetic screens and cancer research, HAP1 offers a streamlined platform for dissecting gene function in hematopoietic malignancy pathways.
CD3E encodes the CD3 epsilon subunit of the T-cell receptor (TCR) complex, essential for TCR surface expression and signal initiation. Upon ligand engagement, Src kinases LCK and FYN phosphorylate CD3E ITAMs, a step modulated by CD45, recruiting ZAP70. ZAP70 phosphorylates LAT and SLP-76, which assemble signaling complexes that activate PLC??1, triggering calcium influx and calcineurin/NFAT activation, along with MAPK, NF-??B, and PI3K-Akt pathways. These cascades drive NFAT, NF-??B, and AP-1 to induce IL-2 and IFN-??. CD3E interacts with CD3D, CD3G, CD247, LCK, FYN, ZAP70, LAT, and GRB2, positioning it upstream of multiple signaling axes.
In the HAP1 haploid background, the CD3E knockout model offers a clean genetic system to probe CD3E-mediated signaling, despite the myeloid origin. The reduced genomic complexity facilitates sensitive detection of interactors and pathway regulators when TCR components are introduced ectopically. This enables haploid genetic screens to identify CD3E modulators or downstream effectors, bridging immunological signaling and cancer research. The knockout population also supports synthetic biology and drug-target validation studies as a controllable loss-of-function platform.
These cells support Western blotting, RT-qPCR, immunofluorescence, flow cytometry, phospho-signaling analysis, and reporter assays. Applications include T-cell biology, immunology, cancer immunotherapy, autoimmune research, functional genomics, and drug screening. They are ideal for antibody validation, high-throughput screening, co-culture experiments, and as negative controls in CD3E-dependent studies. For further information, contact Ascent Research.
