CD46 Knockout NCI-H1299 Polyclonal Cells

Product Type:
Polyclonal Cell Population
Species:
Homo sapiens (Human)
Tissue Source:
Lung
Disease:
Carcinoma

Datasheet

CD46 Knockout NCI-H1299 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population with disrupted CD46 expression in a lung adenocarcinoma background (NCI-H1299; p53 null, EGFR/KRAS wild-type). CD46 functions as a complement regulator via factor I-mediated cleavage of C3b/C4b and as an autophagy inducer through the GOPC-VPS34/Beclin-1 axis. This knockout model eliminates complement protection and impairs autophagy induction, enabling studies on complement-dependent cytotoxicity, viral entry (measles, adenovirus), and CD46-targeted cancer immunotherapy. Suitable for flow cytometry, Western blotting, and functional assays such as complement-mediated cytotoxicity and autophagy flux analysis.

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Cryopreserved in vials and shipped on dry ice

Disclaimer:

For Research Use Only

Characteristics

Host Cell

NCI-H1299

Sex of Donor

Male

Age

43 years

Gene Name

CD46

Gene Identifier

NCBI Gene ID 4179

Morphology

Epithelial-like

Growth Mode

Adherent

Storage

Liquid nitrogen (LN2)
Culture Conditions

Growth medium

RPMI 1640

Supplement(s)

10% Fetal Bovine Serum, 1% Penicillin-Streptomycin Solution

Temperature

37°C

Atmosphere

5% CO₂
Quality Control

Sterility testing

The bacterial, yeast, and fungi are not detected in these cells by daily monitor.

Mycoplasma testing

Negative for mycoplasma through PCR analysis
Disclaimer

Intended Use

This product is intended for laboratory in vitro use only. lt is not intended for diagnostic, therapeutic, or clinical applications.

Disclaimer

Ascent Research endeavors to provide accurate and up-to-date product information. However, no warranties or representations are made regarding its completeness or reliability. References to scientific literature and patents are for informational purposes only, and the customer assumes sole responsibility for verifying their accuracy.

By accepting this product, the customer acknowledges and agrees to assume all risks associated with its receipt, handling, storage, disposal, and use, including compliance with all applicable safety and environmental regulations and precautions. Relevant laws, regulations, and ethical guidelines must be followed in conducting any research, modifications, or derivatives derived from this product.

This product is provided "AS IS", and except as expressly stated herein, Ascent Research disclaims all other warranties, express or implied. Under no circumstances shall Ascent Research, its affiliates, or representatives be liable for indirect, incidental, consequential, or punitive damages arising from the use of this material. While Ascent Research employs rigorous quality control measures, we shall not be held responsible for damages resulting from misidentification or misinterpretation of the provided materials.

Description

This CD46 knockout polyclonal cell population is ideally suited for a range of advanced research applications, including complement evasion and cytotoxicity assays, autophagy flux measurement, mechanistic studies of viral entry for measles and adenovirus, and investigation of CD46 as an immunotherapeutic target.

Researchers can employ techniques such as flow cytometry, Western blotting for autophagic markers like LC3, RT-qPCR, and antibody-dependent complement cytotoxicity assays to characterize functional outcomes.

Additionally, these cells can be used in drug sensitivity screens and migration/invasion assays to explore the role of CD46 in cancer cell biology.

For further technical information or custom inquiries, please contact Ascent Research.