The CD48 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population designed to disrupt the endogenous CD48 gene in HEK293T cells. This product provides a loss-of-function model for investigating CD48-mediated processes without the need for transient knockdown approaches. The polyclonal knockout pool allows rapid generation of CD48-deficient cultures for high-throughput screening and biochemical studies, facilitating consistent target-gene disruption across a mixed cell population.
The host cell line HEK293T is a derivative of human embryonic kidney 293 cells that stably expresses the SV40 large T antigen. This modification enhances episomal replication of plasmids containing the SV40 origin of replication, resulting in high-level protein expression and efficient viral production. HEK293T cells are widely employed for transient transfection, protein?Cprotein interaction studies, and lentiviral packaging due to their robust growth and ease of genetic manipulation. Their epithelial origin and well-characterized signaling networks make them a versatile platform for dissecting receptor-mediated pathways.
CD48 is a glycosylphosphatidylinositol (GPI)-anchored member of the signaling lymphocytic activation molecule (SLAM) family. It serves as a ligand for the receptors CD2 and 2B4 (CD244) and modulates immune cell adhesion and costimulation. Engagement of CD48 by its receptors triggers downstream signaling cascades involving PI3K, AKT, MAPK, and NF-??B. Upstream regulators such as interleukin-2 (IL-2), IL-4, and interferon-gamma control CD48 expression, while its interactions with CD2 and 2B4 recruit Src family kinases Lck and Fyn within lipid rafts, linking receptor activation to transcriptional programs that promote cytokine production and cytotoxicity.
In the HEK293T background, CD48 knockout provides a clean genetic system to study CD48?Creceptor binding and immediate downstream signaling events in the absence of endogenous immune cell context. Because HEK293T cells can be co-transfected with CD2 or 2B4 expression vectors, researchers can reconstitute defined receptor?Cligand pairs to analyze proximal signaling biochemistry. This model is useful for mapping phosphorylation events, protein?Cprotein interactions, and pathway dependencies using immunoprecipitation and reporter assays, without the confounding influences of other SLAM family members typically co-expressed in lymphocytes.
Researchers can use these CD48 knockout HEK293T cells to study CD48-dependent adhesion, screen for modulators of CD48?Creceptor interactions, and dissect NF-??B signaling downstream of CD2/2B4. Applications include flow cytometry, western blotting for PI3K/AKT/MAPK, co-immunoprecipitation with CD2/2B4, and NF-??B reporter assays. For further information, contact Ascent Research.
