CHST3 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal Raji B lymphocyte population with targeted disruption of the CHST3 gene. This heterogeneous pool contains diverse CHST3 loss-of-function mutations, providing a genetically averaged knockout model that minimizes clonal bias. By eliminating chondroitin 6-O-sulfotransferase activity, these cells enable robust functional studies of chondroitin sulfate sulfation in a human B-cell context.
Raji cells are an EBV-positive, Burkitt’s lymphoma-derived lymphoblastoid line exhibiting a mature B-cell phenotype with surface immunoglobulin and classic B-cell markers. Their well-characterized signaling networks and rapid proliferation make them a preferred model for B-cell malignancies and glycobiological investigations. This background is ideal for examining how CHST3 deletion impacts chondroitin sulfate metabolism and lymphoma cell behavior.
The CHST3 gene encodes chondroitin 6-O-sulfotransferase, which catalyzes 6-O-sulfation of GalNAc residues in chondroitin sulfate, generating CS-E and CS-D motifs critical for proteoglycan function. CHST3 acts downstream of regulators SOX9, TGFB1, and BMP2, and partners with CSGALNACT1, CHSY1, and CHPF in a biosynthetic complex. The resultant sulfation patterns dictate proteoglycan interactions with extracellular ligands, influencing cell adhesion, migration, and signaling pathways mediated by chondroitin sulfate proteoglycans such as aggrecan and versican.
In Raji B-lymphoma cells, CHST3-mediated sulfation shapes the tumor cell surface proteoglycan repertoire and microenvironmental interactions. Disruption of CHST3 alters sulfation profiles that may affect cell?Cmatrix adhesion, growth factor binding, and cytokine sequestration, thereby impacting lymphoma progression and immune evasion. This polyclonal knockout model offers a physiologically relevant tool to dissect chondroitin sulfate??s role in malignant B-cell biology and tumor microenvironment crosstalk.
Applications include functional investigation of chondroitin sulfate in B-cell lymphoma using RT-qPCR, Western blot, and HPLC-MS-based disaccharide analysis to assess glycosaminoglycan structure. Immunofluorescence, cell adhesion/migration assays, and flow cytometry allow phenotyping of sulfation changes. The polyclonal population is also suited for high-throughput screening of sulfotransferase inhibitors. For inquiries or customized solutions, please contact Ascent Research.
