The CNTROB Knockout Raji Polyclonal Cells represent a polyclonal population of Raji B lymphocytes engineered via CRISPR/Cas9-mediated disruption of the CNTROB gene. This product delivers a heterogeneous knockout model, preserving the diverse genetic background typical of polyclonal editing outcomes, without clonal selection. The targeted disruption of CNTROB abolishes expression of the centrobin protein, providing a powerful tool for investigating centrosome biology and mitotic regulation in a human B-cell context.
Raji cells, a well-characterized human Burkitt lymphoma-derived B lymphocyte line, exhibit robust proliferative capacity and express surface immunoglobulins, reflecting their antibody-producing and immune surveillance heritage. These cells are widely employed in immunological and cancer research due to their stable growth characteristics and ease of genetic manipulation. Their B-cell lineage makes them particularly suitable for studying centrosome-associated defects in lymphoid malignancies and for evaluating the contribution of mitotic fidelity to lymphomagenesis.
Centrobin, encoded by CNTROB, is a centrosomal protein indispensable for centriole duplication and mitotic spindle assembly. It functions downstream of critical regulators such as PLK4 and CDK2, and cooperates with STIL and SAS-6 to drive procentriole formation. Centrobin interacts with tubulin and various centriolar satellite proteins, facilitating the organization of spindle poles during mitotic progression. Disruption of CNTROB perturbs the PLK4?CSTIL?CSAS-6?CCPAP?CCEP135?Ccentrobin pathway, leading to failed centriole duplication and compromised bipolar spindle formation, ultimately causing mitotic arrest or aberrant chromosome segregation.
In Raji B lymphocytes, centrosomal integrity is vital for accurate mitosis and maintenance of genomic stability. Loss of centrobin function in these lymphoma-derived cells creates a model of centrosome dysfunction that mirrors abnormalities observed in Burkitt lymphoma and other cancers with mitotic instability. The knockout disrupts spindle pole organization, which can lead to abnormal mitotic progression and aneuploidy, providing insight into how centrosomal defects drive oncogenic processes in B-cell malignancies. This system enables dissection of the molecular events linking centrosome duplication and cell cycle control in a disease-relevant hematopoietic background.
Researchers can employ the CNTROB Knockout Raji Polyclonal Cells in a variety of assays to dissect centrosome biology and cancer cell physiology. Immunofluorescence analysis of centrosome markers allows visualization of aberrant centriole structures, while flow cytometry-based cell cycle analysis reveals mitotic arrest or delayed progression. Additional applications include mitotic index scoring, spindle morphology assessment via tubulin staining, proliferation kinetics, and karyotype analysis to evaluate chromosomal instability. These polyclonal knockout cells serve as a versatile resource for investigating gene dependencies and therapeutic vulnerabilities in B-cell lymphoma. For further details, please contact Ascent Research.
