The Dcaf11 Knockout RAW 264.7 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal cell population derived from the murine RAW 264.7 macrophage line, featuring disruption of the Dcaf11 gene. This polyclonal pool provides a heterogeneous knockout model, avoiding clonal selection bias while enabling robust loss-of-function studies. The cells are supplied as a ready-to-use tool for dissecting DCAF11-dependent pathways in a macrophage context.
The RAW 264.7 host line is an Abelson virus-transformed macrophage cell line from BALB/c mice that retains key innate immune functions, including phagocytosis and secretion of inflammatory cytokines such as TNF-?? and IL-6. Its well-characterized signaling networks and genetic tractability make it a standard model for macrophage biology, inflammation, and immune surveillance research.
DCAF11 functions as a substrate receptor for the Cullin4-RING E3 ubiquitin ligase (CRL4) complex, interacting with DDB1, CUL4A/B, and RBX1. It recruits the transcription factor NRF2 (NFE2L2) for ubiquitin-dependent proteasomal degradation, thus negatively regulating the antioxidant response. Under oxidative stress or electrophile challenge, DCAF11-mediated degradation is inhibited, allowing NRF2 to accumulate and activate antioxidant response element (ARE)-driven genes, including NQO1, HMOX1, and GCLM. Dcaf11 knockout therefore stabilizes NRF2 and constitutively induces antioxidant gene expression.
In RAW 264.7 macrophages, loss of DCAF11 disrupts redox homeostasis and can alter inflammatory responses due to crosstalk between NRF2 and NF-??B pathways. This polyclonal knockout model permits examination of how sustained NRF2 activation affects phagocytosis, ROS handling, and cytokine secretion, providing insights into macrophage regulation in oxidative stress-related conditions such as cancer and neurodegeneration.
These cells are suited for a variety of assays, including western blotting for DCAF11 and NRF2, RT-qPCR of Nqo1 and Hmox1, ARE-luciferase reporter assays, and ROS measurement. They also enable co-immunoprecipitation studies of CRL4 complex interactions and functional assays for phagocytic activity and inflammatory cytokine production. Applications include NRF2 pathway investigation, ubiquitin-proteasome research, macrophage redox biology, and screening for NRF2 modulators. For further information, contact Ascent Research.
