The DCPS Knockout THP-1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population designed to disrupt the DCPS gene in the human THP-1 monocytic cell line. This product provides a loss-of-function model for studying the role of DCPS, a scavenger decapping enzyme critical in mRNA decay pathways. The polyclonal population preserves genetic heterogeneity, enabling studies of gene function without the clonal biases associated with single-cell-derived lines. This knockout model is useful for investigating the effects of DCPS on gene expression and cellular responses.
The THP-1 cell line was established from the peripheral blood of a one-year-old male with acute monocytic leukemia (FAB M5). THP-1 cells are widely used as a model for monocytes and macrophages, particularly for studying inflammatory and immune responses. They exhibit characteristics such as phagocytic activity and the ability to differentiate into macrophage-like cells upon stimulation with phorbol esters. This well-characterized line provides a robust platform for examining monocyte biology, signal transduction, and host-pathogen interactions.
DCPS is a scavenger decapping enzyme that hydrolyzes the 5′ m7G cap of short mRNA fragments after 3′??5′ exonucleolytic decay. DCPS depletion causes accumulation of capped oligoribonucleotides, impacting global mRNA stability and gene regulation. It functions within the mRNA surveillance pathway and interacts with exosome subunits (e.g., EXOSC10) and the exonuclease XRN1. Downstream effects include altered levels of pro-inflammatory cytokines TNF-?? and IL-6. Related pathway factors include DCP1A, DCP2, the Lsm1-7 complex, and PNPT1, which collectively coordinate RNA turnover.
In the context of THP-1 monocytes, DCPS deficiency disrupts normal mRNA decay, potentially leading to prolonged mRNA half-lives and aberrant inflammatory gene expression. This KO model enables investigation of how impaired cap hydrolysis influences monocyte activation, differentiation, and cytokine production. Given links between DCPS and neurological disorders, combined with the immune-modulatory properties of THP-1, this system may also be applied to study neuroimmune crosstalk or drug effects via transcriptomic analysis.
The DCPS Knockout THP-1 Polyclonal Cells are suitable for diverse research applications including studying mRNA turnover kinetics with actinomycin D chase assays, measuring cytokine secretion via ELISA, and profiling global transcriptomic changes by RNA-seq. These cells can be utilized in drug sensitivity and inhibitor screening studies targeting the decapping machinery, as well as in mechanistic investigations of DCPS-associated signaling in immune cells. Standard validation assays include Western blotting, RT-qPCR, and cap-hydrolysis enzymatic assays. For personalized support or to order this product, please contact Ascent Research.
