The DIP2A Knockout HT29 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human HT29 colorectal adenocarcinoma line, featuring targeted disruption of the DIP2A gene. As a polyclonal pool, these cells provide a heterogeneous loss-of-function model suitable for population-level phenotypic analyses of DIP2A-dependent processes in intestinal epithelial biology and colorectal cancer.
The host HT29 cell line originates from a primary colorectal adenocarcinoma of a 44-year-old female and is a well-established model for intestinal epithelial cell biology. HT29 cells exhibit absorptive and secretory functions and can form polarized monolayers, making them valuable for studies of barrier integrity, cell adhesion, and transport. Their cancerous origin underpins their widespread use in colorectal cancer research, including tumorigenesis, metastasis, and drug response studies.
DIP2A encodes a protein that interacts with Disabled-2 (DAB2) and functions in Frizzled receptor signaling, modulating Wnt/planar cell polarity (PCP) and Hippo pathways. It is regulated by WNT ligands such as WNT3A and microRNA MIR-433. DIP2A forms complexes with DAB2, Frizzled receptors, and clathrin adaptor AP2, and its disruption influences the activity of Rho GTPases RAC1 and RHOA and Hippo effectors YAP and TAZ. As a result, DIP2A loss can perturb cell polarity, endocytic trafficking, and transcriptional programs governing proliferation.
In HT29 colorectal cancer cells, DIP2A knockout provides a model to dissect how disruption of Wnt/PCP and Hippo signaling affects malignant phenotypes. The polarized epithelial character of HT29 cells makes them ideal for examining DIP2A??s role in cell polarity and directional migration, key processes in invasion and metastasis. Additionally, DIP2A??s interaction with clathrin and AP2 suggests a role in receptor trafficking, potentially modulating oncogenic Wnt signaling. These polyclonal cells are thus valuable for research into colorectal and gastric cancers.
Researchers can utilize these polyclonal knockout cells in Western blotting to assess DIP2A depletion and changes in DAB2, RAC1, and phospho-YAP. RT-qPCR enables profiling of Wnt/PCP target genes. Immunofluorescence staining for polarity markers (e.g., ZO-1) can reveal planar polarity alterations. Migration, invasion, and proliferation assays are directly applicable for studying metastatic behavior. Co-immunoprecipitation can map protein complexes involving DAB2 and Frizzled receptors. For further details, contact Ascent Research.
