The DIS3L2 Knockout A2780 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population generated from the A2780 human ovarian carcinoma epithelial line. These cells contain a heterogeneous pool of DIS3L2 gene disruptions, creating a robust loss-of-function model for studying RNA metabolism. As a polyclonal knockout pool, the product offers a representative sample of knockout-induced genetic variation, suitable for population-level analyses. The CRISPR/Cas9-mediated gene disruption yields a mixed genotype that effectively abolishes DIS3L2 protein expression across the culture.
The A2780 cell line is an established model of ovarian endometrioid adenocarcinoma, derived from an untreated patient. These epithelial cells retain key features of the original tumor and are widely used in cancer research for studies of tumorigenesis, drug response, and gene regulation. They provide a disease-relevant system for exploring the role of RNA decay in ovarian cancer biology.
DIS3L2 encodes an exoribonuclease that degrades uridylated RNAs, critical for mRNA surveillance and miRNA biogenesis. It is activated by terminal uridylyltransferases TUT4 (ZCCHC11) and TUT7 (ZCCHC6), which add uridine tails to target RNAs. LIN28A indirectly affects this by modulating let-7 uridylation. Key substrates include uridylated let-7 precursors, mRNAs, and ncRNAs. DIS3L2 thus forms a functional axis with TUT4 and TUT7 in the uridylation-mediated decay pathway, regulating gene expression post-transcriptionally. This pathway is crucial for normal RNA turnover and its disruption has implications in cancer.
In the A2780 context, loss of DIS3L2 disrupts uridylation-dependent RNA decay, potentially altering let-7 miRNA processing and mRNA stability. Given associations with Perlman syndrome and Wilms tumor, this model aids in dissecting DIS3L2??s tumor-suppressive roles and how RNA metabolism aberrations drive cancer. It is ideal for studying impacts on proliferation, apoptosis, and drug sensitivity in ovarian cancer.
These polyclonal knockout cells are suitable for miRNA processing studies, RNA decay mechanism investigations, and transcriptome-wide analyses. Key assays include RT?qPCR for let-7 levels to monitor miRNA processing, RNA stability assays to measure decay rates of uridylated transcripts, western blotting to validate DIS3L2 protein depletion, RNA?seq to identify global transcriptomic changes, and reporter assays for functional analysis of mRNA decay. For further details and ordering information, please contact Ascent Research.
