The DIXDC1 Knockout NCI-H1299 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population derived from the human lung adenocarcinoma cell line NCI-H1299, harboring disruption of the DIXDC1 gene. This loss-of-function model allows investigation of DIXDC1-dependent signaling without clonal artifacts. The cells retain parental NCI-H1299 characteristics, including adherent growth and standard culture conditions. As a polyclonal pool, it offers robust and reproducible functional analyses.
The NCI-H1299 cell line is a human non-small cell lung adenocarcinoma originally established from a lymph node metastasis. It is widely used as a model for studying NSCLC biology, including proliferation, invasion, and drug response. The adherent epithelial cells provide a relevant genetic background for oncogenic signaling research and are particularly suited for investigating pathways involved in metastatic dissemination. Their use in xenograft models further extends their utility for in vivo evaluation of tumorigenicity.
DIXDC1 is a scaffold protein that positively regulates canonical WNT/??-catenin signaling by bridging Dishevelled (DVL2, DVL3) and AXIN1. This assembly inhibits the ??-catenin destruction complex (AXIN1, APC, GSK3B), leading to ??-catenin (CTNNB1) stabilization and nuclear translocation, where it partners with TCF7L2 to activate target genes like MYC, CCND1, and AXIN2. DIXDC1 functions downstream of WNT ligands (WNT1, WNT3A) through Frizzled and LRP6 receptors, and it intersects with the PI3K/AKT pathway, integrating growth and survival cues.
In NCI-H1299 lung cancer cells, DIXDC1 knockout enables dissection of WNT/??-catenin-driven oncogenic mechanisms. Researchers can assess impacts on ??-catenin stabilization, target gene expression, and phenotypes such as proliferation, anchorage-independent growth, and migration. The model is valuable for studying the scaffold??s role in tumorigenesis, EMT, and drug sensitivity, as well as for in vivo studies of metastatic potential and tumor microenvironment interactions.
These cells support a broad range of assays, including TOP/FOP reporter, western blot for ??-catenin and MYC, RT-qPCR for CCND1 and AXIN2, colony formation, and Transwell migration. Co-IP of AXIN1-DVL2 and ??-catenin immunofluorescence further probe pathway integrity. Applications extend to drug sensitivity profiling and xenograft tumorigenicity. For further information, please contact Ascent Research.
