The DLAT Knockout MES-OV Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population derived from the MES-OV mouse ovarian mesenchymal cell line, featuring targeted disruption of the Dlat gene. This heterogeneous population lacks functional DLAT protein, serving as a loss-of-function model for studying DLAT-dependent processes. The polyclonal format preserves genetic diversity, making it suitable for metabolic flux and drug response assays where population-level behavior is critical.
MES-OV cells are fibroblast-like mesenchymal stromal cells derived from mouse ovary. They contribute to ovarian tissue architecture through extracellular matrix production, paracrine signaling, and immunomodulation. Their ovarian origin provides a relevant model for exploring stromal-epithelial interactions, ovarian cancer microenvironments, and metabolic regulation in reproductive tissues.
DLAT encodes the E2 subunit of the pyruvate dehydrogenase complex (PDC), which converts pyruvate to acetyl-CoA, linking glycolysis to the TCA cycle. It interacts with PDHA1, PDHB, DLD, and PDHX to form the catalytic core. Activity is regulated by PDK and PDP kinases/phosphatases, responding to insulin, nutrients, and hypoxia via HIF-1??. DLAT disruption impairs PDC assembly, reducing mitochondrial respiration and promoting a glycolytic shift. This rewires acetyl-CoA-dependent processes including ATP synthesis, histone acetylation, and lipid biosynthesis, mirroring metabolic adaptations in cancer and neurodegeneration.
In ovarian mesenchymal MES-OV cells, DLAT knockout forces a shift from oxidative phosphorylation to glycolysis, altering the metabolic profile of the stroma. This can modulate secretory factors, matrix remodeling, and support for adjacent epithelial cells, offering insights into ovarian cancer metabolic crosstalk, endometriosis, and stromal cell differentiation. The model allows dissection of how metabolic reprogramming influences mesenchymal cell function and tumor microenvironment dynamics.
These polyclonal knockout cells are useful for Seahorse metabolic flux analysis, PDH activity assays, and western blotting of PDC subunits. Additional applications include RT-qPCR of metabolic genes, acetyl-CoA quantification, immunofluorescence for mitochondrial markers, and proliferation assays under metabolic stress. Contact Ascent Research for further information.
