DLG1 Knockout HeLa Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population targeting DLG1 in the HeLa human cervical epithelial line. This loss-of-function model, generated via CRISPR/Cas9-mediated gene disruption, maintains allelic diversity suitable for population-level phenotypic studies without clonal selection bias. It serves as a robust platform for investigating DLG1-dependent processes, including cell polarity, tight junction biology, and tumor suppression.
The HeLa cell line originates from a cervical adenocarcinoma of Henrietta Lacks. HPV18-positive, these cells express viral oncoproteins E6 and E7, driving transformation. Their epithelial morphology and extensive characterization make HeLa cells a staple in cancer biology and signal transduction research, providing a relevant background for examining tumor suppressor genes like DLG1 in oncogenic contexts.
DLG1 is a MAGUK scaffold protein critical for tight junction assembly and cell polarity. It interacts with APC, PTEN, LIN7, and CASK, coupling the actin cytoskeleton to junctional complexes. DLG1 participates in the Hippo pathway by modulating MST1/2, LATS1/2, and YAP/TAZ, and in the Wnt pathway by scaffolding APC and ??-catenin to restrain ??-catenin transcriptional activity. The HPV E6 oncoprotein targets DLG1 for degradation, linking viral oncogenesis to polarity loss. Thus, DLG1 disruption compromises tight junction integrity and deregulates Hippo and Wnt/??-catenin signaling, potentially enhancing PI3K/AKT pathway activity.
In HeLa cells, DLG1 knockout recapitulates E6-mediated degradation, driving HPV-associated carcinogenesis. Loss of DLG1 disrupts epithelial barrier function, promotes invasive behavior, and activates proliferative signals. This model enables dissection of DLG1’s tumor suppressor role in an HPV-positive background, facilitating studies on migration, invasion, and transformation. The polyclonal format allows examination of heterogeneous responses and compensatory mechanisms following gene disruption.
Research applications include western blotting and immunofluorescence for protein analysis, co-immunoprecipitation for interaction networks, and transwell assays for migration/invasion. Barrier function can be assessed via TEER measurement, and pathway activities via luciferase reporters for Wnt and Hippo signaling. The cells are relevant for HPV oncogenesis, neurodevelopmental disorder models, and cell polarity studies. For more information or custom requests, contact Ascent Research.
