The DLG1 Knockout MES-OV Polyclonal Cells product consists of a CRISPR/Cas9-edited polyclonal knockout cell population targeting the murine Dlg1 gene in the MES-OV embryonic stem cell line. This polyclonal knockout model provides a genetically heterogeneous pool of cells with disrupted DLG1 expression, enabling robust loss-of-function studies across a range of experimental contexts. The product is designed for researchers investigating the scaffolding functions of DLG1 in cell signaling, polarity, and differentiation without introducing clonal biases.
The host cell line, MES-OV, is a well-characterized mouse embryonic stem cell line derived from the 129/Ola strain. These pluripotent stem cells maintain the capacity for self-renewal and can differentiate into derivatives of all three germ layers??endoderm, mesoderm, and ectoderm??making them an ideal model system for developmental biology, stem cell research, and in vitro differentiation assays. The 129/Ola background provides a stable genetic platform for gene targeting and has been widely used in generating knockout models.
DLG1 encodes a membrane-associated guanylate kinase (MAGUK) scaffold protein that orchestrates the assembly of multi-protein complexes at cell?Ccell junctions and synapses. At adherens junctions, DLG1 links cadherins to the actin cytoskeleton through direct interactions with beta-catenin and APC, while in the Wnt signaling pathway it modulates signal transduction by scaffolding APC, beta-catenin, AXIN, and GSK-3beta, ultimately influencing TCF/LEF transcriptional activity. DLG1 function is regulated upstream by Src kinase-mediated phosphorylation, CaMKII phosphorylation, cell adhesion signals, and Wnt ligands, and it impacts downstream targets including Kv1.5 ion channels, AMPA receptors, and cytoskeletal components. Additionally, DLG1 interacts with CASK, LIN7, DLGAP1, and NMDA receptors, contributing to synaptic organization and neurotransmitter receptor clustering.
Disruption of DLG1 in the MES-OV pluripotent stem cell context is particularly valuable for dissecting its roles in self-renewal, lineage commitment, and early developmental processes. As a scaffold integrating adhesion and signaling inputs, DLG1 likely influences pluripotency maintenance and directed differentiation through its control of cell polarity and pathway crosstalk. The polyclonal knockout population allows assessment of phenotypic variability and threshold effects that may be masked in clonal lines, making it suitable for studies of cancer stem cell behavior, neuroectodermal specification, and synapse formation in differentiating neurons.
Typical research applications include immunofluorescence-based analysis of junctional protein localization, co-immunoprecipitation of DLG1-interacting partners such as APC, beta-catenin, and ion channels, and western blotting or RT-qPCR to examine downstream gene expression changes. Functional assays may involve Wnt reporter luciferase measurements, migration or invasion studies in cancer biology contexts, and electrophysiological recordings to assess ion channel function. In differentiation protocols, the knockout cells can be used to evaluate requirements for germ layer formation or neuronal maturation. For additional information or to request a quotation, please contact Ascent Research.
