The DLG3 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal HEK293T population carrying targeted disruptions in the DLG3 gene. DLG3 encodes a membrane-associated guanylate kinase (MAGUK) scaffold that organizes postsynaptic signaling complexes. This heterogeneous knockout pool enables robust loss-of-function studies without clonal artifacts and is suitable for biochemical, cell biological, and pharmacological investigations.
HEK293T cells are an immortalized human embryonic kidney line stably expressing SV40 large T antigen, which facilitates high-level protein production and viral packaging. Their ease of transfection and ability to heterologously express neuronal proteins make them an ideal system for analyzing synaptic scaffolding proteins like DLG3 in a controlled, non-neuronal background.
DLG3 assembles postsynaptic complexes by binding through its PDZ domains to glutamate receptor subunits GRIN2A, GRIN2B, GRIA1, and GRIA2, and to scaffold partners DLG4, DLGAP1, and SHANK1, coupling receptors to the cytoskeleton. Its function is modulated by glutamate, NMDA receptor activation, and CaMKII phosphorylation, which regulate receptor trafficking and clustering. Knockout of DLG3 disrupts these interactions, enabling analysis of PDZ domain specificity and scaffold dynamics.
In the HEK293T context, the DLG3 polyclonal knockout cells offer a simplified system to study receptor clustering, protein?Cprotein interactions, and trafficking without endogenous synaptic complexity. Eliminating this central MAGUK protein allows researchers to map interaction domains, evaluate receptor surface expression dependencies, and screen for small-molecule modulators. The polyclonal format enhances statistical robustness and minimizes single-clone bias in assays such as co-immunoprecipitation, immunofluorescence, and FRET.
Applications include co-immunoprecipitation and Western blotting for interaction mapping, immunofluorescence to assess receptor clustering, FRET for live-cell complex analysis, and electrophysiology with co-expressed receptors. This model is valuable for investigating DLG3-linked neurodevelopmental disorders such as intellectual disability and schizophrenia. Contact Ascent Research for support.
