The DLG3 Knockout HeLa Polyclonal Cells provide a CRISPR/Cas9-edited polyclonal population of HeLa cells with targeted disruption of the human DLG3 gene, which encodes the SAP102 scaffolding protein. This polyclonal format consists of a heterogeneous pool of gene-edited cells, suitable for loss-of-function studies without clonal isolation. The CRISPR/Cas9-mediated gene disruption ablates SAP102 expression, enabling investigation of its roles in signal transduction and cellular organization.
HeLa cells are an extensively characterized human cervical carcinoma line derived from HPV18-positive adenocarcinoma, featuring inactivation of the p53 and Rb tumor suppressors, high proliferation rates, and an aneuploid karyotype. Their epithelial origin and robust growth characteristics make them a standard model for cancer biology, cell signaling, and cytoskeletal studies, providing a relevant context for assessing the non-neuronal functions of the DLG3-encoded scaffold protein.
As a MAGUK family member, SAP102 organizes postsynaptic density-like complexes by scaffolding NMDA receptor subunits (GluN1, GluN2), adaptor proteins (PSD-95, GKAP, Shank), and tumor suppressors APC and PTEN. It is activated by NMDA receptor signaling and phosphorylated by CaMKII, Src kinases, and PKC, and mediates downstream Wnt/??-catenin pathway activation through ??-catenin stabilization and TCF/LEF transcriptional regulation. SAP102 also interacts with Kir2.3 channels and cytoskeletal components (actin, spectrin), thereby coupling synaptic-like signaling to cell polarity and adhesion.
In the HeLa epithelial background, DLG3 knockout disrupts the SAP102-anchored network that links Wnt/??-catenin signaling, cell adhesion, and polarity. Since SAP102 associates with the tumor suppressors APC and PTEN??both commonly dysregulated in cervical cancers??its loss may alter ??-catenin-dependent transcription, cytoskeletal dynamics, and migratory behavior. This model thus enables dissection of how a synaptic scaffold protein contributes to cancer-relevant processes independently of neuronal context.
The DLG3 knockout HeLa cells support applications including Western blot confirmation of protein loss, co-immunoprecipitation of interacting partners, immunofluorescence localization studies, Wnt reporter assays to measure ??-catenin activity, and functional assays for proliferation and migration. RT-qPCR can monitor transcriptional changes in targets like ??-catenin and TCF/LEF. These cells are suited for screening compounds that modulate SAP102-mediated pathways and for comparative studies of MAGUK family function in cancer cell biology. For further details, contact Ascent Research.
