The DLG5 Knockout HeLa Polyclonal Cells provide a CRISPR/Cas9-edited polyclonal knockout cell population targeting the human DLG5 gene in HeLa cells. This loss-of-function model enables investigation of DLG5-dependent mechanisms in an immortalized, epithelial background. The polyclonal nature reflects diverse editing outcomes across the cell pool, offering a robust system for studying gene disruption effects without single-cell cloning bias.
HeLa cells, derived from a cervical adenocarcinoma, are widely used as an epithelial cancer model. They harbor integrated HPV18 sequences that produce the E6 oncoprotein, which targets p53 for degradation, thereby impairing cell cycle control and apoptosis. This immortalized line provides a consistent genetic background for studying cancer-related pathways and epithelial biology.
DLG5 encodes a scaffold protein essential for cell polarity and tight junction assembly. It functions downstream of PRKCA and ROCK kinases and Wnt ligands, forming complexes with PARD3, AMOT, and CTNNB1. DLG5 modulates Hippo signaling by influencing YAP/TAZ localization, and interacts with CTNNB1 to fine-tune Wnt transcriptional responses via TCF/LEF factors. Knockout of DLG5 disrupts these interactions, leading to altered Hippo pathway activity and compromised tight junction integrity.
In the HeLa background, loss of DLG5 removes a key polarity determinant while p53 remains inactivated, creating a model to dissect polarity-dependent tumor suppressive mechanisms independent of p53. The knockout cells exhibit diminished junctional scaffolding, potentially affecting YAP/TAZ and CTNNB1 signaling. This enables study of how DLG5 orchestrates crosstalk between the Hippo and Wnt pathways in an oncogenic context.
This polyclonal knockout population is suitable for assays including Western blotting, immunofluorescence, co-immunoprecipitation, migration, and proliferation studies. Applications include investigating cancer cell polarity, Hippo pathway regulation, and drug sensitivity testing. The cells provide a versatile tool for functional genomics and signaling interrogation. For more information, please contact Ascent Research.
