The DLG5 Knockout HGC-27 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population that enables functional studies of the DLG5 gene in a gastric carcinoma context. These cells were generated by disrupting DLG5 in the HGC-27 human gastric cancer cell line, yielding a heterogeneous pool of gene-edited variants ideal for studying loss-of-function phenotypes without clonal selection biases.
The HGC-27 cell line originates from a signet ring cell gastric carcinoma derived from a lymph node metastasis, maintaining characteristics of epithelial cells from the gastric mucosa. This model retains key features of gastric cancer, including alterations in cell polarity, adhesion, and invasive potential, making it a suitable host for investigating genes involved in epithelial integrity and tumor suppression.
At the molecular level, DLG5 acts as a scaffold that orchestrates tight junction assembly by tethering ZO-1, occludin, and claudins to F-actin, while also stabilizing adherens junctions through interactions with E-cadherin, ??-catenin, and ??-catenin. It functions downstream of epithelial polarity cues and Wnt/PCP components, and its loss leads to defective junctional complex formation, increased paracellular permeability, and potential derepression of YAP/TAZ transcriptional activity. DLG5 also interfaces with the LKB1?CAMPK energy-sensing pathway, implicating it in cellular responses to metabolic stress.
Given the origin of HGC-27 from a metastatic gastric carcinoma, DLG5 knockout in this background allows dissection of how polarity loss contributes to invasive behavior. Studies have linked DLG5 to inflammatory bowel disease and colorectal cancer, and its disruption may promote oncogenic signaling through Hippo pathway dysregulation. The polyclonal nature enables evaluation of heterogeneous responses, recapitulating the diversity seen in tumor cell populations.
Typical applications include monitoring tight junction integrity via TEER and permeability assays, analyzing protein expression and localization by Western blotting and immunofluorescence, and assessing cell migration and invasion in 2D and 3D culture systems. Co-immunoprecipitation can validate protein interactions, while RT-qPCR and RNA-seq enable transcriptional profiling. The polyclonal population provides a robust system for drug screening campaigns aimed at restoring epithelial barrier function or inhibiting metastasis. For technical inquiries, please contact Ascent Research.
