The DLGAP4 Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population engineered to disrupt the DLGAP4 gene, providing a loss-of-function model in a human T lymphoblastoid background. These polyclonal cells maintain population heterogeneity and are suitable for studying bulk DLGAP4-dependent processes in T cell biology without the bias of single-cell cloning.
Jurkat cells are an immortalized human T lymphoblastoid line derived from peripheral blood of an acute T cell leukemia patient. They grow as suspension cultures and serve as a standard model for T cell receptor (TCR) signaling, T cell activation, and leukemogenic pathway analysis. This background offers a well-characterized platform for probing immune synapse formation and T cell-mediated responses in both physiological and pathophysiological contexts.
DLGAP4 encodes a scaffolding protein that organizes postsynaptic density complexes in neurons, interacting with DLG4/PSD95 and SHANK proteins. In Jurkat T cells, it likely participates in immune synapse assembly by linking TCR signaling components to the actin cytoskeleton. Its activity is regulated by TCR engagement and Src family kinases, and it forms complexes with DLG1, DLG4/PSD95, SHANK1, and SHANK3. Downstream, DLGAP4 promotes actin cytoskeleton remodeling and influences cytokine production, integrating signals from proximal TCR effectors such as ZAP70 and LAT to coordinate cytoskeletal dynamics and T cell effector functions.
Disruption of DLGAP4 in this model is particularly relevant for investigating the immune dimensions of neurodevelopmental disorders, given DLGAP4’s association with autism spectrum disorder, schizophrenia, and intellectual disability. In the Jurkat background, the model also enables exploration of how scaffolding proteins may impact leukemic signaling, offering insights into T cell leukemia biology and potential cross-talk between neuronal and immune synapse organization.
This polyclonal knockout pool is applicable to a range of assays, including Western blotting and RT-qPCR for knockout validation, co-immunoprecipitation to assess protein interactions with DLG1 or SHANKs, immunofluorescence to visualize immune synapse formation, and flow cytometry to quantify T cell activation markers and cytokine secretion. Applications include screening for regulators of T cell signaling, drug discovery for T cell-mediated diseases, and investigating neurodevelopmental gene function in immune cells. For more information or to place an order, please contact Ascent Research.
