The DLGAP4 Knockout MES-OV Polyclonal Cells product supplies a CRISPR/Cas9-edited polyclonal knockout cell population in which the gene encoding SAPAP4 (DLGAP4) has been disrupted in the MES-OV mouse ovarian surface epithelial cell line. This polyclonal pool contains a mixed population of edited cells, providing a loss-of-function model for studying DLGAP4-dependent processes in an epithelial context without clonal selection artifacts. The knockout population is suited for experiments requiring bulk functional analysis of DLGAP4 ablation, such as signaling pathway interrogation and tumorigenicity assays.
The MES-OV cell line is a spontaneously immortalized mouse ovarian surface epithelial line that maintains a non-tumorigenic phenotype. It recapitulates key features of ovarian surface epithelium, which undergoes cyclic wound repair and proliferation during ovulation, providing a relevant model for studying early events in ovarian neoplastic transformation.
DLGAP4 encodes SAPAP4, a scaffold protein that couples DLG/MAGUK family members (e.g., DLG1/SAP97, DLG4/PSD-95) to the actin cytoskeleton at epithelial junctions. SAPAP4 also recruits SHANK proteins and ??-catenin into junctional complexes, thereby organizing signaling centers. Its expression is regulated by Wnt ligands, EGF/EGFR, TGF-??, and integrin-mediated adhesion, which trigger downstream effectors including JNK/MAPK signaling and the stabilization of tight junction (claudin, occludin) and adherens junction (E-cadherin/??-catenin) components. Importantly, DLGAP4 intersects with the Hippo pathway by influencing the localization and activity of Scribble, LATS1/2, and YAP/TAZ, linking cell?Ccell adhesion to growth control.
Disruption of DLGAP4 in MES-OV cells likely compromises junctional integrity and polarity by uncoupling MAGUK proteins from the cytoskeleton, impairing Hippo and Wnt/??-catenin signaling. This leads to deregulated YAP/TAZ and ??-catenin activity, mimicking early tumorigenic changes. The non-tumorigenic nature of the line allows investigation of cooperative oncogenic events required for full transformation.
This DLGAP4 polyclonal knockout cell population is well-suited for a broad range of advanced research applications, including quantitative analysis of DLGAP4 expression by Western blotting and RT-qPCR, visualization of junctional markers (E-cadherin, ZO-1) via immunofluorescence, and functional assays such as cell adhesion, transwell migration/invasion, and proliferation measurements. Additionally, it supports mechanistic studies of Hippo-Wnt crosstalk using phospho-YAP detection and Wnt reporter assays. Researchers investigating ovarian epithelial homeostasis, epithelial-to-mesenchymal transition (EMT), or screening adhesion-targeted therapeutics will find this model a powerful tool for elucidating DLGAP4??s role in normal and pathological states. For further information, please contact Ascent Research.
