The DLGAP4 Knockout Raji Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal cell population derived from Raji B lymphocytes, carrying a targeted disruption of the DLGAP4 gene. This knockout model offers a loss-of-function system for investigating the scaffold protein DLGAP4 in a human immune cell background. The polyclonal nature minimizes clonal bias while enabling robust analysis of gene function across a genetically heterogeneous pool.
Raji cells are a human Burkitt lymphoma-derived B lymphocyte line extensively used in immunology and oncology research. As a lymphoblastoid cell type, Raji maintains characteristic B cell markers and signaling pathways, making it suitable for studying B cell adhesion, activation, and migration. The line??s reliable growth and assay compatibility further support detailed molecular and cellular investigations.
DLGAP4 functions as a scaffold linking DLG family members (e.g., DLG4/PSD95, DLG1) and Shank proteins to the actin cytoskeleton via cortactin and Rho GTPases. It plays key roles in cell adhesion and postsynaptic organization, interacting with NMDA receptor subunits (GluN1/GluN2) in neurons. Upstream signals including cell adhesion cues and potentially Wnt signaling regulate DLGAP4, which in turn modulates actin reorganization and adhesion complex dynamics. In B lymphocytes, DLGAP4 likely orchestrates similar cytoskeletal events at immune synapses and cell?Ccell contacts.
Disruption of DLGAP4 in Raji cells is expected to compromise cytoskeletal integrity and adhesion, affecting processes such as B cell migration and antigen recognition. This model provides insights into scaffold protein functions in lymphoma biology, including potential roles in malignant transformation and metastasis. By ablating DLGAP4 expression, researchers can delineate its contributions to B cell receptor signaling and mechanotransduction.
Applications include adhesion, migration, and invasion assays to dissect DLGAP4-dependent cellular dynamics. Standard validation methods like Western blotting, RT-qPCR, and immunofluorescence can confirm knockout and assess downstream effects, while flow cytometry reveals immune phenotype changes. Co-immunoprecipitation and phosphoproteomic approaches further map altered signaling networks. This product is a versatile tool for advancing knowledge in immunology and cancer cell biology. For further inquiries, please contact Ascent Research.
