The DLGAP5 Knockout K-562 Polyclonal Cells represent a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human K-562 lymphoblastoid leukemia cell line. This product offers a genetically disrupted DLGAP5 locus, generating a robust loss-of-function model for investigating the mitotic functions of DLGAP5 (also known as HURP). The polyclonal knockout pool minimizes artifacts associated with single-cell clonal selection, enabling functional studies of spindle assembly and cell cycle regulation in a leukemic background.
K-562 is a widely characterized cell line established from the pleural effusion of a patient with Philadelphia chromosome-positive chronic myelogenous leukemia (CML) in blast crisis. As a suspension lymphoblastoid line, K-562 serves as a standard model for CML and for studying BCR-ABL-driven oncogenic signaling, providing a clinically relevant host for dissecting mitotic vulnerabilities in leukemia.
DLGAP5 is a mitotic spindle assembly factor that stabilizes kinetochore microtubules, essential for proper chromosome alignment and segregation. The protein is recruited to spindle microtubules via a Ran GTPase-dependent mechanism and activated through Aurora A-mediated phosphorylation. Upstream regulation involves E2F transcription factors and the TPX2?CAurora A kinase module, with importin ?? further modulating localization. Downstream, DLGAP5 influences microtubule dynamics, mitotic progression, and genome stability. It functions within a core spindle regulatory network including Ran GTPase, TPX2, Aurora A, and importin ??, linking mitotic signaling to the fidelity of chromosome segregation.
In the K-562 CML background, dysregulated cell cycle control driven by the BCR-ABL oncogene creates a unique context to examine how spindle assembly defects contribute to leukemic proliferation. DLGAP5 is overexpressed in multiple malignancies??including hepatocellular carcinoma, breast cancer, and lung adenocarcinoma??and portends poor prognosis. Disruption of DLGAP5 in a leukemic model may uncover synthetic lethal interactions with oncogenic signaling or enhance sensitivity to spindle-targeting chemotherapeutics, offering a platform for translational studies.
The DLGAP5 Knockout K-562 Polyclonal Cells are designed for experimental applications such as mechanistic dissection of mitotic spindle assembly, functional analysis of chromosome segregation, identification of synthetic lethal relationships, and preclinical validation of mitotic drug targets. Recommended approaches include Western blotting to confirm DLGAP5 loss, immunofluorescence imaging of spindle morphology and chromosome alignment, flow cytometry for cell cycle distribution and apoptosis, and cell proliferation assays. For further information, please contact Ascent Research.
