The DLGAP5 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population generated from the human Raji B-lymphocyte cell line, with disruption of the DLGAP5 gene to provide a loss-of-function model. This heterogeneous pool bypasses clonal selection, offering a versatile system to study DLGAP5 (HURP) functions in lymphoid cells. The CRISPR/Cas9-mediated gene disruption enables investigation of mitotic processes and cancer biology without the constraints of single-cell isolation.
Raji is an EBV-positive Burkitt’s lymphoma B lymphocyte line harboring the t(8;14) translocation that drives MYC overexpression. It serves as a well-established model for B-cell lymphoma and antibody production studies. The high proliferative rate and defined genetic background of Raji cells make them particularly suited for examining cell cycle?Crelated genes such as DLGAP5.
DLGAP5 encodes a microtubule-associated protein essential for mitotic spindle assembly and chromosome segregation. It localizes to kinetochore fibers and central spindle, where it stabilizes microtubules in a Ran-GTP?Cdependent manner. Aurora A kinase phosphorylates DLGAP5, promoting interaction with TPX2 and KIF11. Upstream, FOXM1 transcriptionally regulates DLGAP5 expression, while Ran GTPase and Aurora A signaling control its activity. DLGAP5 interacts with importin beta and forms complexes with TPX2 and microtubules, functioning downstream of the Ran?CAurora A signaling axis. Loss of DLGAP5 disrupts mitotic spindle integrity and cell cycle progression.
In Raji cells, DLGAP5 knockout likely compromises mitotic fidelity, potentially inducing cell cycle arrest or apoptosis. DLGAP5 is often overexpressed in cancers including B-cell lymphoma and hepatocellular carcinoma, correlating with poor outcomes. This polyclonal knockout model, therefore, offers a physiologically relevant system to study how DLGAP5-dependent spindle regulation sustains lymphoma cell proliferation, especially under MYC-driven growth conditions. It allows exploration of mitotic vulnerabilities that could be targeted therapeutically.
The DLGAP5 Knockout Raji Polyclonal Cells are amenable to diverse applications, including immunofluorescence microscopy for spindle morphology assessment, western blotting, flow cytometry for cell cycle analysis, RT-qPCR, and proliferation or apoptosis assays. They support research into mitotic spindle assembly, cell cycle regulation, cancer cell proliferation, and drug target validation in B-cell lymphoma models. For additional technical details or custom services, please contact Ascent Research.
