DMRT2 Knockout HAP1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population engineered for the targeted disruption of the DMRT2 gene in the HAP1 human haploid cell line. This ready-to-use loss-of-function model enables the study of DMRT2, a member of the Doublesex and mab-3 Related Transcription factor family, which plays roles in sexual development and segmentation. The polyclonal nature of the knockout population provides a heterogeneous pool of edited cells, facilitating robust functional assays without the need for clonal isolation.
The HAP1 cell line originates from the KBM-7 chronic myeloid leukemia (CML) line, derived from a patient in blast crisis. As a near-haploid cell line, HAP1 contains a single copy of most chromosomes, making it an exceptional platform for CRISPR/Cas9-mediated genome editing. A single guide RNA targeting the allele of interest can generate a functional knockout, eliminating the need for biallelic targeting. This haploid genetic background is highly valued for high-throughput genetic screening, loss-of-function phenotyping, and studies requiring clear genotype-phenotype correlations.
DMRT2 encodes a transcription factor implicated in vertebrate sexual development and segmentation. Within the sex determination cascade, DMRT2 may be regulated by SRY, SOX9, and NR5A1, and interacts with DMRT1, DMRT3, and NR5A1. Downstream targets potentially include SOX9, AMH, and CYP19A1, placing it at a critical node in gonadogenesis. It helps coordinate genes required for proper gonadal differentiation.
The haploid HAP1 model offers a unique advantage for studying DMRT2. Although of hematopoietic origin, it provides a simplified genetic context free from diploid redundancy. DMRT2 disruption here enables clear assessment of transcriptional targets and signaling partners, and is useful for mapping regulatory networks and conducting haploid genetic screens for synthetic lethality or pathway modulators.
This knockout population supports functional characterization of DMRT2, dissection of sex determination, and genome-wide haploid genetic screening. Assays include RT-qPCR and western blot for validation, RNA-seq for transcriptomics, ChIP-qPCR for target identification, and immunofluorescence for localization. For further information, please contact Ascent Research.
