The DMWD Knockout HAP1 Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout cell population targeting the DMWD gene in the near-haploid HAP1 cell line. This heterogeneous pool carries diverse loss-of-function alleles, providing a robust tool for functional studies without clonal bias. The product is designed for applications ranging from genetic screening to mechanistic dissection of myotonic dystrophy type 1-associated pathways.
HAP1 is a male adherent cell line derived from KBM-7 chronic myeloid leukemia cells, with a near-haploid karyotype that facilitates unambiguous gene disruption. Its leukemic origin and stable adhesion support high-content imaging and biochemical analyses, while preserving signaling pathways relevant to neuromuscular research. The near-haploid state minimizes genetic redundancy, enabling clear genotype-phenotype correlations in loss-of-function experiments.
DMWD encodes a WD40 repeat-containing protein located at the myotonic dystrophy type 1 (DM1) locus, adjacent to DMPK. It is thought to function as a scaffold in protein complexes, potentially interacting with DMPK, MBNL1, CELF1, and spliceosomal components. DMWD may share regulatory elements with DMPK, and its dysregulation is linked to DM1 pathogenesis. The protein participates in RNA processing and protein-protein interaction networks, making it a critical node for investigating multisystemic features of myotonic dystrophy.
In the HAP1 background, loss of DMWD creates an isogenic model to interrogate its specific function in DM1-related mechanisms. The near-haploid state reduces dominant masking effects, allowing clear detection of DMWD-dependent phenotypes such as altered splicing or DMPK kinase activity. This platform facilitates genetic interaction studies between DMWD and DMPK, and enables screening for modifiers of disease pathways, complementing patient-derived models.
Key applications include functional genomics screens (CRISPR dropout, drug sensitivity), co-immunoprecipitation for protein interaction mapping, immunofluorescence, RT-qPCR, RNA-seq, and DMPK kinase reporter assays. The polyclonal nature ensures phenotypic robustness across diverse mutations, providing a reliable resource for both targeted assays and large-scale studies. For further technical details or ordering information, please contact Ascent Research.
