The DNAAF2 Knockout HGC-27 Polyclonal Cells product is a CRISPR/Cas9-edited polyclonal knockout cell population designed for loss-of-function studies of the DNAAF2 gene in the HGC-27 human gastric carcinoma cell line. DNAAF2 encodes a dynein axonemal assembly factor required for the cytoplasmic pre-assembly of axonemal dynein arms, the molecular motors driving ciliary motility. This polyclonal pool provides a heterogeneous knockout population, avoiding clonal artifacts and enabling robust functional analyses in a cancer-relevant cellular context.
The HGC-27 host cell line is an epithelial cell line derived from a metastatic lymph node of a gastric cancer patient. It is a well-characterized model for gastric carcinoma research, widely used to investigate proliferation, migration, and invasion. HGC-27 cells are capable of forming primary cilia under defined conditions, making them valuable for studying ciliary biology and its intersection with tumor progression.
DNAAF2 functions in a pre-assembly pathway regulated by the ciliogenic transcription factors FOXJ1 and RFX. In the cytoplasm, it interacts with DNAAF1 (LRRC50) to facilitate the stable assembly of dynein heavy chains, including DNAH5 and DNAH11, into functional dynein arm complexes. Other key pathway members include dynein intermediate chains DNAI1 and DNAI2. Disruption of DNAAF2 blocks outer dynein arm formation, impairing ciliary beat frequency and motility.
In the HGC-27 gastric cancer context, DNAAF2 knockout disrupts ciliary function, which can modulate cell migration and invasion??processes linked to ciliary signaling. This polyclonal model enables the study of cilia-dependent mechanisms in gastric cancer progression and serves as a tool for investigating primary ciliary dyskinesia-associated phenotypes in a non-classical cell background.
This knockout population is suited for diverse applications, including ciliary function assays, disease modeling, and gastric cancer motility studies. Relevant readouts include immunofluorescence for ciliary markers (acetylated ??-tubulin, ARL13B), high-speed video microscopy for ciliary beat frequency, western blotting for dynein components (DNAH5, DNAI1), migration/invasion transwell assays, and RT-qPCR for ciliogenesis genes. For inquiries, please contact Ascent Research.
