The DNAH5 Knockout Jurkat Polyclonal Cells are a CRISPR/Cas9-edited human T-lymphocyte population featuring targeted disruption of the DNAH5 gene. This polyclonal pool contains a heterogeneous array of editing events, offering a robust loss-of-function model without clonal isolation. Derived from the Jurkat E6-1 acute lymphoblastic leukemia line, these cells provide a versatile platform for studying DNAH5 in a non-ciliated background.
Jurkat cells are an immortalized T-cell leukemia model extensively used to investigate T-cell receptor signaling, apoptosis, and immune cell function. As these cells lack motile cilia, DNAH5 knockout does not induce ciliary dyskinesia. Instead, the model serves as an ideal ciliary-negative control or a system to explore non-ciliary roles of the axonemal dynein heavy chain.
DNAH5 encodes an outer dynein arm heavy chain that functions as an ATPase motor driving ciliary beat. In ciliated cells, its expression is regulated by transcription factors FOXJ1, RFX2, RFX3, and MCIDAS, with negative input from NOTCH signaling. The protein interacts with DNAI1, DNAI2, DNAH11, CCDC114, and ARMC4 to form the outer dynein arm complex. Loss of DNAH5 results in immotile cilia, defective mucociliary clearance, and left-right axis disruption. In Jurkat cells, this knockout reconstructs a loss-of-function context for studying dynein heavy chain biology independent of ciliary structures.
The absence of cilia in Jurkat cells repositions the DNAH5 knockout model toward applications in off-target screening, antibody validation, and genomic integrity studies. The polyclonal nature minimizes clonal bias and provides a heterogeneous population suitable for bulk analyses. It also serves as a critical non-ciliary control for experiments requiring ciliary-negative backgrounds, such as compound screening or pathway analyses where ciliary effects must be excluded.
Investigators can utilize these cells in viability, apoptosis, and proliferation assays to assess DNAH5 targeting effects. Genomic PCR and Sanger sequencing confirm editing at the DNAH5 locus, while RT-qPCR and western blot enable quantification of transcript and protein levels. Immunocytochemistry can monitor the localization of dynein components. This product is well-suited for antibody validation, off-target analysis, and as a negative control in ciliary motility studies. For further technical specifications or custom inquiries, please contact Ascent Research.
