The DNAH5 Knockout MES-OV Polyclonal Cells are a CRISPR/Cas9-edited polyclonal cell population derived from MES-OV, an ovarian clear cell carcinoma line, with targeted disruption of the DNAH5 gene. This heterogeneous pool contains diverse knockout genotypes, reducing clonal bias and enabling robust population-level functional studies. The gene disruption generates a loss-of-function model for dissecting DNAH5-dependent biological processes.
The MES-OV host cell line originates from a human ovarian clear cell carcinoma, an epithelial cancer subtype characterized by distinct molecular features. It serves as a clinically relevant in vitro model for investigating ovarian cancer biology, including mechanisms of metastasis, drug resistance, and tumor microenvironment interactions.
DNAH5 encodes an outer dynein arm heavy chain essential for ciliary and flagellar motility. Its transcription is regulated by FOXJ1 and RFX transcriptional factors (RFX2, RFX3). The DNAH5 protein interacts with DNAI1, DNAI2, and DNAL1 to form the force-generating dynein complex, driving ATP-dependent microtubule sliding and ciliary beat. Downstream, ciliary motility underpins mucociliary clearance, cerebrospinal fluid flow, and left-right axis determination. In primary cilia, Hedgehog signaling relies on intact ciliary architecture: Hedgehog binding to PTCH1 activates SMO, leading to GLI transcription factor activation. Loss of DNAH5 may impair ciliary motility and potentially alter Hedgehog signal transduction.
Within the ovarian cancer context, ciliary dysfunction has been linked to tumor progression and altered signaling. These DNAH5 knockout MES-OV cells allow dissection of how ciliary beat deficiency influences cancer-relevant phenotypes such as migration, invasion, and response to Hedgehog pathway inhibitors. The model enables correlation of ciliary motility with downstream signaling outputs, including GLI activation, and exploration of crosstalk with Wnt/planar cell polarity pathways, offering insights into cilia-dependent mechanisms in ovarian clear cell carcinoma.
Key applications include studying ciliary biology, modeling primary ciliary dyskinesia, and assessing mucociliary clearance defects. Researchers can employ western blotting for DNAH5 expression, immunofluorescence for cilia markers (acetylated tubulin, ARL13B), ciliary beat frequency measurement by high-speed microscopy, RT-qPCR for ciliogenesis genes, RNA-seq transcriptomics, and functional assays such as cell cycle analysis, apoptosis, and migration/invasion. The polyclonal format supports both targeted mechanistic studies and broader phenotypic screens. For further details or to request a quote, contact Ascent Research.
