The DNAJA2 Knockout A2780 Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout cell population derived from the A2780 human ovarian carcinoma cell line. This product offers a heterogeneous pool of gene-disrupted cells, enabling population-level studies of DNAJA2 loss without the confounding effects of single-cell clonal selection. It serves as a versatile loss-of-function model for dissecting DNAJA2-dependent processes in protein homeostasis and stress response pathways.
The A2780 cell line, originally established from an ovarian adenocarcinoma patient, is a widely employed epithelial model for ovarian cancer research. These adherent cells retain critical oncogenic characteristics and exhibit sensitivity to endoplasmic reticulum (ER) stress, making them highly suitable for investigating proteostasis and chaperone biology. The A2780 background provides a cancer-relevant context that enhances the translational significance of DNAJA2 functional studies.
DNAJA2 encodes a J-domain co-chaperone that potently stimulates Hsp70 ATPase activity, thereby driving the folding, trafficking, and degradation of client proteins. It functions within the Hsp70?CHOP?CCHIP chaperone network, linking molecular chaperones to the ubiquitin-proteasome system for misfolded protein clearance. Under stress, HSF1 transcriptionally upregulates DNAJA2, integrating heat shock and ER stress signals. Consequently, DNAJA2 knockout impairs Hsp70-mediated quality control, leading to accumulation of aberrant proteins and heightened sensitivity to proteotoxic insults.
In the A2780 ovarian cancer model, disruption of DNAJA2 is expected to intensify basal proteotoxic stress and compromise cellular resilience to ER stress-inducing chemotherapeutics. Given the dependence of cancer cells on robust protein quality control, this polyclonal knockout model reveals vulnerabilities in chaperone networks and provides a platform to identify synthetic lethal interactions with proteasome inhibitors or other stress-modulating agents. Such studies may inform novel therapeutic strategies targeting proteostasis in ovarian and other cancers.
These knockout cells are ideally suited for Western blotting to validate DNAJA2 and Hsp70 levels, proteotoxicity assays employing fluorescent aggregation reporters, and viability assays under chemically induced stress. Co-immunoprecipitation experiments enable probing of Hsp70?Cco-chaperone complex composition, while ubiquitin-proteasome activity measurements directly assess degradation pathway function. Researchers can utilize this model to explore protein homeostasis, screen pharmacological modulators of the heat shock response, and evaluate DNAJA2 as a potential therapeutic target. For further technical details or to request a quotation, please contact Ascent Research.
