The DNAJA2 Knockout HeLa Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population with targeted disruption of the DNAJA2 gene. This pooled format avoids clonal selection, enabling studies of DNAJA2 loss in a heterogeneous cell population typical of biological systems. CRISPR/Cas9-mediated gene disruption creates a loss-of-function model for investigating DNAJA2-dependent cellular processes.
HeLa cells are an immortalized human cervical adenocarcinoma epithelial cell line extensively used in cancer research and studies of cellular stress. Their robust growth and well-characterized signaling networks make them a suitable host for examining co-chaperone functions in oncogenesis and proteostasis.
DNAJA2 is a J-domain co-chaperone that stimulates the ATPase activity of Hsp70 chaperones (HSPA8/Hsc70 and HSPA1A/Hsp70), regulating protein folding, mitochondrial import, and degradation. It acts downstream of HSF1 and cellular stress signals, and interacts with mitochondrial import receptors Tom70 and Tim23. Through modulation of Hsp70, DNAJA2 influences mitochondrial protein translocation and apoptotic regulators, functioning within a network that includes Hsp90, CHIP, and BAG co-chaperones.
In HeLa cells, DNAJA2 knockout compromises Hsp70-mediated proteostasis, impairing mitochondrial protein import and increasing apoptosis susceptibility. This is particularly relevant for cancer models, as HeLa cells depend on chaperone systems to manage oncogenic stress. The knockout may sensitize cells to mitochondrial toxins or proteasome inhibitors, providing insights into protein quality control and cell death pathways.
Applications include western blotting for DNAJA2 and Hsp70, mitochondrial fractionation and import assays, Annexin V apoptosis measurements, co-immunoprecipitation of Hsp70 complexes, RT-qPCR of stress-responsive genes, and cell viability assays. This product supports research in cancer biology, neurodegeneration, and drug sensitivity. For ordering or technical inquiries, please contact Ascent Research.
