This product is a CRISPR/Cas9?edited polyclonal K?562 cell population carrying targeted disruption of the DNAJA2 gene, enabling loss?of?function analysis of this cochaperone. The polyclonal format preserves heterogeneous editing outcomes, avoiding clonal artifacts and supporting robust functional studies. These cells provide a ready?to?use model for investigating DNAJA2??s role in proteostasis within a hematopoietic context.
The K?562 host line, derived from a CML patient in blast crisis, is a Philadelphia chromosome?positive lymphoblast capable of spontaneous differentiation into erythrocytic, granulocytic, and monocytic lineages. Widely applied in leukemia biology and hematopoietic differentiation studies, its suspension growth and high transfectability facilitate genetic perturbation and pharmacological screening.
DNAJA2 acts as a J?domain cochaperone that stimulates Hsp70 (HSPA1A) ATP hydrolysis, stabilizing Hsp70?client complexes to drive protein folding, trafficking, and degradation. It is transcriptionally upregulated by HSF1 and ER stress sensors (IRE1, PERK, ATF6). Within the chaperone network, DNAJA2 interacts with HSPH1, BAG3, and the E3 ligases STUB1 (CHIP) and Parkin, dictating the fate of clients such as mutant p53, Tau, ???synuclein, and CFTR. This positions DNAJA2 as a key node linking stress signaling to the ubiquitin?proteasome system.
In K?562 cells, DNAJA2 disruption offers insight into how cochaperone function influences leukemic phenotypes. Because K?562 rely on Hsp70 for survival and can undergo multilineage differentiation, loss of DNAJA2 may destabilize oncogenic clients, alter differentiation trajectories, or enhance chemosensitivity. This model is therefore valuable for studying the unfolded protein response in CML and for evaluating Hsp70?targeted therapeutics.
These polyclonal knockout cells are suited for cancer biology, protein misfolding, drug resistance, and neurodegenerative disease modeling. Assays such as HSE?luciferase reporter, co?immunoprecipitation with Hsp70, Annexin V apoptosis staining, and cell cycle analysis enable comprehensive phenotypic characterization. Used alongside Western blotting, these tools facilitate dissection of DNAJA2?dependent pathways. For support, contact Ascent Research.
