The DNAJB12 Knockout MES-OV Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the MES-OV human ovarian endometrioid carcinoma cell line. They feature targeted disruption of the DNAJB12 gene, which encodes an ER-associated degradation (ERAD) co-chaperone. The polyclonal editing generates a heterogeneous loss-of-function pool, enabling robust genotype-phenotype studies without clonal isolation. This product offers a versatile system for investigating DNAJB12-dependent processes in an ovarian cancer epithelial context.
MES-OV is an epithelial cancer cell line originating from ovarian endometrioid carcinoma. It serves as a clinically relevant model for studying ovarian cancer biology, including tumor progression and stress responses. In these cells, the DNAJB12 knockout allows dissection of how ERAD co-chaperone activity impacts cancer cell protein homeostasis and survival signaling.
DNAJB12 is a J-domain co-chaperone that collaborates with Hsp70 to recognize and retrotranslocate misfolded ER proteins via the ERAD pathway. Its activity is modulated by upstream ER stress transducers ATF6 and IRE1, which initiate UPR signaling. DNAJB12 forms complexes with Hsp70 and VCP/p97, extracting substrates from the ER membrane and targeting them for proteasomal degradation. Loss of DNAJB12 disrupts this clearance mechanism, leading to accumulation of misfolded proteins and chronic ER stress.
In MES-OV cells, DNAJB12 knockout likely impairs ERAD, heightening ER stress and UPR activation. Since ovarian cancer cells often rely on robust protein folding to sustain proliferation, this deficiency may expose vulnerabilities in quality control networks. Additionally, DNAJB12 is linked to neurodegeneration, including Parkinson??s disease, making this model valuable for exploring ERAD dysfunction across cancer and neurobiology.
This polyclonal knockout is optimized for ER stress profiling via Western blot (BiP, CHOP) and RT-qPCR of UPR targets. Researchers can assess protein aggregation by immunofluorescence and measure cell viability under chemical ER stress. The model also facilitates studies of DNAJB12 interactions with Hsp70, VCP/p97, and the proteasome, and supports drug screening for ERAD modulators. For further details or to order this product, please contact Ascent Research.
