DNAJB14 Knockout HGC-27 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the HGC-27 human gastric carcinoma cell line. This heterogeneous pool of cells carries targeted disruptions in the DNAJB14 gene, providing a versatile loss-of-function model for studying DNAJB14-dependent biology in a cancer-relevant background. The polyclonal nature reflects a population-level gene knockout suitable for assays that do not require clonal homogeneity.
The HGC-27 host cell line originates from a poorly differentiated human gastric adenocarcinoma and is widely employed as an in vitro model of gastric epithelial tumor biology. HGC-27 cells retain characteristics of advanced gastric carcinoma, making them particularly useful for investigating oncogenic signaling, tumor cell adaptation, and therapeutic response in the context of gastric cancer.
DNAJB14 encodes a J-domain co-chaperone that plays a critical role in endoplasmic reticulum-associated degradation (ERAD). It recruits misfolded ER proteins to the degradation machinery by interacting with key components such as HSPA5 (BiP), DERL1, VIMP, and the p97/VCP complex. This function is activated by upstream unfolded protein response (UPR) sensors and transcription factors, including ATF6, XBP1, and ATF4. DNAJB14-mediated substrate targeting promotes the dislocation and subsequent proteasomal degradation of aberrant proteins, thereby maintaining ER proteostasis and mitigating ER stress-induced cytotoxicity.
In gastric adenocarcinoma, dysregulation of ER protein quality control pathways is increasingly recognized as a contributor to tumor survival and drug resistance. As a model of poorly differentiated gastric cancer, HGC-27 cells may rely on DNAJB14 to sustain ER homeostasis under the heightened secretory and proliferative demands of malignant cells. Disruption of DNAJB14 in this background thus offers a valuable system to probe how tumor cells cope with chronic ER stress and to identify potential therapeutic vulnerabilities.
This polyclonal knockout cell population is well suited for a range of research applications, including identification of DNAJB14-dependent ERAD substrates, dissection of ER stress signaling pathways, and analysis of tumor cell adaptation to proteotoxic insults. Representative assays include western blotting for DNAJB14 and ER stress markers, RT-qPCR for UPR target genes, immunofluorescence to assess ER morphology and protein localization, co-immunoprecipitation with HSPA5 to probe protein interactions, and viability assays under proteasome inhibition or ER stress induction. Researchers can use these cells to study the functional consequences of DNAJB14 loss in gastric cancer progression and drug sensitivity. For further technical specifications or to place an order, please contact Ascent Research.
