The DNAJB14 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the Raji B-cell line, providing a pooled loss-of-function model for studying DNAJB14. The polyclonal format comprises a heterogeneous mixture of cells with distinct gene disruptions, avoiding clonal bias and enabling direct population-level functional analyses. This ready-to-use product eliminates the need for single-cell cloning, facilitating scalable experimental workflows.
Raji cells, originating from Burkitt lymphoma, are Epstein-Barr virus (EBV)-positive suspension B lymphocytes widely used as a model for B-cell malignancies and viral latency. Their rapid growth and well-defined signaling make them an ideal host for investigating gene function in a disease-relevant context.
DNAJB14 functions as an Hsp40 co-chaperone that recruits Hsp70 to client proteins, regulating protein folding and clathrin-dependent endosomal trafficking. It interacts with the AP-2 adaptor complex, sorting nexins, and co-chaperones HIP, CHIP, and HOP, while nucleotide exchange factors BAG and HspBP1 modulate the cycle. Expression is controlled by HSF1 and unfolded protein response sensors, linking stress signaling to trafficking. Downstream, DNAJB14 influences the trafficking of endocytic cargo receptors such as EGFR and transferrin receptor, thereby intersecting signal transduction and membrane dynamics.
In the Raji B-cell context, DNAJB14 knockout is expected to disrupt endocytic sorting and protein homeostasis, potentially impairing receptor signaling, cell proliferation, and survival. As EBV-positive lymphoma cells, they may additionally reveal roles for DNAJB14 in viral latency maintenance, given the virus’s exploitation of host chaperone machinery. This system thus offers a platform to dissect co-chaperone-dependent mechanisms in lymphomagenesis and EBV biology.
The knockout cells are suitable for functional assays including transferrin uptake endocytosis measurements, flow cytometry for surface receptor dynamics (EGFR, transferrin receptor), cell proliferation and apoptosis assays, co-immunoprecipitation with Hsp70 or clathrin, and RT-qPCR/Western blotting of client gene expression. They are also valuable for drug screening targeting the Hsp70 chaperone network or endosomal trafficking in B-cell lymphoma. For further information, contact Ascent Research.
