The DNAJC13 Knockout Huh-7 Polyclonal Cells constitute a CRISPR/Cas9-edited polyclonal knockout population derived from Huh-7 hepatocellular carcinoma cells, with targeted disruption of the DNAJC13 gene. This polyclonal format provides a heterogeneous loss-of-function model for investigating DNAJC13??s role in endosomal trafficking, avoiding the selection bias of single-cell clones. These cells are designed for advanced biomedical research into trafficking-related pathologies.
Huh-7 is a well-differentiated hepatocarcinoma line from a Japanese male, permissive to hepatitis C virus (HCV) and extensively used as a hepatic metabolism and liver cancer model. Its high endocytic activity and HCV susceptibility make it an ideal system to study how DNAJC13-dependent sorting influences hepatocyte functions, receptor recycling, and lysosomal homeostasis in a disease-relevant context.
DNAJC13 (RME-8) recruits Hsc70 to endosomal clathrin coats, driving clathrin uncoating and enabling retromer (VPS35/VPS26/VPS29)-mediated sorting of cargo receptors such as Sortilin, CI-MPR, and Wntless back to the TGN or plasma membrane. It interacts directly with clathrin, sorting nexins (SNX1/SNX2), and the WASH complex, and is regulated by Rab5, Rab7, LRRK2, and EGFR signaling. DNAJC13 loss disrupts retromer function, leading to receptor mis-sorting, lysosomal degradation defects, and aberrant Wnt pathway activation??pathways central to neurodegenerative diseases like Parkinson??s disease 21 and endosomal trafficking disorders.
In Huh-7 cells, DNAJC13 knockout models impaired endosomal-lysosomal trafficking that is critical for liver cancer cell survival, autophagy regulation, and HCV lifecycle. The polyclonal nature enables studies under heterogeneous editing backgrounds, better mimicking in vivo tissue complexity. This system facilitates dissection of how trafficking perturbations contribute to both hepatocarcinogenesis and neurodegeneration, bridging liver biology and neuropathology.
Researchers can employ these cells for endosomal sorting assays (transferrin uptake, EGFR recycling), confocal imaging of clathrin dynamics, immunofluorescence detection of endolysosomal markers (EEA1, Rab5, LAMP1), and autophagy flux analysis (LC3 turnover). Additional applications include lysosomal enzyme activity measurements, co-chaperone function assays, and drug screening for trafficking modulators. Wound healing assays further enable evaluation of DNAJC13??s impact on hepatocellular carcinoma cell migration. For detailed product specifications, please contact Ascent Research.
