DNAJC19 Knockout Raji Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population derived from the human Burkitt??s lymphoma B-cell line Raji. This product provides a heterogeneous pool of cells carrying target-gene disruptions, enabling loss-of-function studies of the mitochondrial co-chaperone DNAJC19 in a lymphoid cell context. The knockout model supports investigations into mitochondrial protein import and cristae organization.
The Raji host cell line is an immortalized B-lymphocyte model established from a patient with EBV-positive Burkitt??s lymphoma. These suspension cells express characteristic B-cell surface markers CD19 and CD20, and they are extensively employed in B-cell biology and lymphomagenesis research. Their metabolic profile provides a relevant platform to examine mitochondrial processes in malignant B cells.
DNAJC19 functions as a J-domain co-chaperone in the mitochondrial matrix, where it interacts with HSPA9 (mitochondrial Hsp70) and stimulates its ATPase activity to facilitate preprotein translocation through the TIM23 complex. Key interacting factors include PAM16, TIMM23, TIMM17A, and TIM44. Upstream regulators such as PGC-1?? and NRF1 control mitochondrial biogenesis, while downstream targets comprise HSPA9 and TIM23 subunits. Disruption of DNAJC19 impairs the chaperone cycle, leading to defective oxidative phosphorylation and altered cristae morphology.
In the Raji B-cell model, DNAJC19 knockout links mitochondrial protein import defects to lymphocyte physiology. This system is particularly relevant for studying dilated cardiomyopathy with ataxia (DCMA) and broader mitochondrial disorders, while offering insights into metabolic vulnerabilities of proliferating lymphoma cells. Impaired TIM23 function may affect B-cell receptor signaling, apoptosis, and immune effector functions.
Research applications include mechanistic dissection of TIM23-dependent import, modeling DCMA syndrome, and screening for modulators of mitochondrial chaperones. The cells are amenable to Western blotting for complex components, co-immunoprecipitation of HSPA9, Seahorse stress tests, immunofluorescence for TOM20, flow cytometric TMRE measurements, RT-qPCR of biogenesis markers, and apoptosis assays. For additional information, please contact Ascent Research.
