The DNAJC25 Knockout SK-HEP-1 Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout cell population with targeted disruption of the DNAJC25 gene. This heterogeneous pool of SK-HEP-1 cells serves as a loss-of-function model to study the gene’s role without the selective pressures of clonal isolation, making it suitable for experiments requiring population-level genetic diversity.
The SK-HEP-1 parental line is a human hepatic adenocarcinoma cell line with endothelial characteristics, derived from ascites of a hepatocellular carcinoma patient. It recapitulates features of liver sinusoidal endothelial cells, enabling research on hepatic vascular function and tumor microenvironment interactions. This unique model bridges cancer biology and endothelial cell studies within the liver.
DNAJC25 encodes a DnaJ co-chaperone that partners with Hsp70 family proteins, including HSPA1A and HSPA5/BiP, to mediate protein folding and ER quality control. It functions within the unfolded protein response (UPR) network, regulated by ATF6, IRE1??/XBP1, and PERK/eIF2??/ATF4 pathways. Disruption of DNAJC25 compromises Hsp70 chaperone complexes, impairs the clearance of misfolded proteins via ERAD, and sensitizes cells to ER stress-induced apoptosis, highlighting its pivotal role in maintaining proteostasis.
In the SK-HEP-1 background, DNAJC25 knockout is particularly informative for dissecting how co-chaperone dysfunction influences hepatocellular carcinoma progression and endothelial cell responses. The model can be used to investigate ER stress-mediated effects on cancer cell proliferation, migration, and drug resistance, as well as on endothelial barrier function and tumor angiogenesis. These studies are relevant to understanding liver cancer biology and ER stress-related disorders.
Typical applications include western blotting for DNAJC25 and UPR markers, RT-qPCR for UPR target genes, immunofluorescence for ER stress markers, and flow cytometry for apoptosis. The cells are also suited for migration/invasion assays and drug sensitivity screens with ER stress inducers. For more information or to place an order, contact Ascent Research.
