The DNAJC7 Knockout HEK293T Polyclonal Cells are a CRISPR/Cas9-edited polyclonal knockout population disrupting the DNAJC7 gene in HEK293T cells. This heterogeneous pool, generated via CRISPR/Cas9-mediated gene disruption, provides a loss-of-function model for studying DNAJC7 biology. The polyclonal format captures diverse mutational events without clonal isolation, avoiding clone-specific artifacts and enabling robust population-level analyses.
HEK293T cells are a widely used derivative of human embryonic kidney HEK293 cells, stably expressing the SV40 large T antigen for episomal plasmid replication and high transfection efficiency. This epithelial cell line supports efficient recombinant protein expression and gene editing, making it an ideal chassis for investigating chaperone networks, ubiquitin-mediated proteolysis, and signaling pathways in a human cellular background.
DNAJC7 encodes a co-chaperone that associates with HSC70 (HSPA8) and HSP70 (HSPA1A) to mediate ATP-dependent protein folding, trafficking, and degradation. It interacts with BAG2 and STUB1 to target clients for proteasomal elimination and modulates the tumor suppressor p53, influencing cell cycle arrest and apoptosis. DNAJC7 is transcriptionally regulated by HSF1, p53, ATF6, and IRE1, integrating proteotoxic stress signaling with chaperone-mediated protein quality control. Downstream apoptosis regulators BAX and BCL2 are also impacted, linking DNAJC7 to cell survival decisions.
In HEK293T, DNAJC7 knockout uncouples its specific contributions to chaperone activity and p53 regulation. Loss of DNAJC7 can sensitize cells to proteotoxic stress and alter apoptotic thresholds, providing a platform to study how co-chaperone pathways interface with tumor suppressor networks. The high-transfection competency of HEK293T facilitates rescue experiments and reporter-based readouts, while the polyclonal nature ensures representation of multiple knockout events, yielding a comprehensive functional picture.
This product enables diverse assays: co-immunoprecipitation for chaperone complex analysis, p53 reporter assays, proteasome activity measurements, and flow cytometric apoptosis profiling. It is suitable for heat shock response studies, functional genomics screens, and drug discovery targeting chaperone or p53 pathways in cancer and neurodegeneration. For further information, please contact Ascent Research.
