The DNAL1 Knockout AGS Polyclonal Cells are a CRISPR/Cas9-edited polyclonal population derived from the AGS human gastric adenocarcinoma cell line, engineered for loss-of-function studies of the DNAL1 gene. DNAL1 encodes an axonemal dynein light chain essential for outer dynein arm assembly and ciliary motility. This polyclonal knockout model provides a heterogeneous genetic background ideal for phenotypic screening, avoiding clonal selection biases. It serves as a powerful tool to dissect DNAL1-dependent processes in a gastric epithelial context, leveraging the well-characterized AGS cell line.
The AGS cell line is a widely used model of gastric mucosal epithelium, retaining mucin expression and receptor profiles relevant to gastric function and disease. Although derived from an adenocarcinoma, AGS cells exhibit differentiated features enabling cilia-related investigations. CRISPR-mediated DNAL1 disruption in this background permits exploration of ciliary roles in gastric homeostasis, host-pathogen interactions, and tumor biology.
DNAL1 functions as a dynein light chain within the outer dynein arm, interacting with heavy chains (DNAH5, DNAH11) and intermediate chains (DNAI1, DNAI2), as well as DNALI1 and docking complex subunits. Its expression is regulated by FOXJ1 and RFX transcription factors. DNAL1 is critical for dynein motor activity and ciliary beat frequency; its knockout disrupts mucociliary clearance and axonemal complex assembly. In AGS cells, this knockout provides a tractable system to study interactions essential for motile cilia function.
In gastric epithelium, ciliated cells contribute to luminal sensing and mucus clearance. DNAL1 knockout mirrors defects seen in primary ciliary dyskinesia and Kartagener syndrome, offering a model to investigate ciliopathy mechanisms and gastric-specific phenotypes. Researchers can explore how ciliary dysfunction affects gastric mucosal responses, including altered mucin secretion or susceptibility to H. pylori infection, bridging genetic defects and epithelial pathophysiology.
These polyclonal knockout cells support a range of assays, including immunofluorescence for ciliary markers, co-immunoprecipitation for dynein complex interactions, ciliary motility measurements, and electron microscopy for ultrastructure. They are suitable for primary ciliary dyskinesia research, screening for ciliopathy modulators, and studying cilia-dependent signaling in gastric cells. Typical techniques include Sanger sequencing, Western blotting, RT-qPCR, and functional motility assays. For further information or technical support, please contact Ascent Research.
